DNA/RNA Oxidative Damage EIA Kit
Cayman Chemical Item Number 589320
Limit of detection: 80% B/B0: ~30 pg/ml · Sensitivity: 50% B/B0: ~100 pg/ml · Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of DNA/RNA oxidative damage that detects all three oxidized guanine species; 8-
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Baldridge, K.C., Zavala, J., Surratt, J., et al. Cellular RNA is chemically modified by exposure to air pollution mixtures. Inhal Toxicol 27 74-82 (2015).
Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).
Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).
Gan, W., Nie, B., Shi, F., et al. Age-
Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-
Roszkowski, K., and Olinski, R. Urinary 8-
Weimann, A., Broedbaek, K., Henriksen, T., et al. Assays for urinary biomarkers of oxidatively damaged nucleic acids. Free Radic Res 46(4) 531-540 (2012).
Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-
Pricing updated 2015-04-25. Prices are subject to change without notice.
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Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample DNA/RNA Oxidative Damage EIA Kit under the Cayman Challenge program.
Let us analyze your samples for you. See our contract research services.
The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?
It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.Show answer Hide answer
Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?
The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.Show answer Hide answer
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