DNA/RNA Oxidative Damage EIA Kit

Cayman Chemical Item Number 589320

8-OH-2dG; 8-OH-DG; 8-hydroxy-2'-Deoxyguanosine; DNA/RNA Oxidative Damage ELISA

DNA/RNA Oxidative Damage EIA Kit

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Description

Limit of detection: 80% B/B0: ~30 pg/ml · Sensitivity: 50% B/B0: ~100 pg/ml · Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of DNA/RNA oxidative damage that detects all three oxidized guanine species; 8-hydroxy-2'-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from either DNA or RNA. Some commercial vendors offer immunoassays that detect only 8-hydroxy-2'-deoxyguanosine, and not the other two molecules. The advantage of the Cayman kit is that it captures a more complete set of biologically relevant products of oxidative damage than do assays that are restricted to analysis of only 8-hydroxy-2'-deoxyguanosine. However, because Cayman’s kit recognizes more than 8-hydroxy-2'-deoxyguanosine, it is not valid to compare the results from the Cayman EIA Kit to an LC/MS analysis of 8-hydroxy-2'-deoxyguanosine. The EIA value will always be significantly higher than LC/MS because the EIA also detects 8-hydroxyguanosine and 8-hydroxyguanine.
Synonyms
  • 8-OH-2dG
  • 8-OH-DG
  • 8-hydroxy-2'-Deoxyguanosine
  • DNA/RNA Oxidative Damage ELISA
Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
8-hydroxy-2’-deoxyguanosine 100%
8-hydroxyguanosine 38%
8-hydroxyguanine 23%
Guanosine <0.01%
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Background Reading

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Weimann, A., Broedbaek, K., Henriksen, T., et al. Assays for urinary biomarkers of oxidatively damaged nucleic acids. Free Radic Res 46(4) 531-540 (2012).

Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy. Cancer Epidemiol Biomarkers Prev 21 629-634 (2012).

Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging. Free Radic Res 46(9) 1093-1098 (2012).

Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging. Free Radic Biol Med 52 1700-1707 (2012).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

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589320-96SOLID
DNA/RNA Oxidative Damage AChE Tracer 100 dtn
DNA/RNA Oxidative Damage EIA Monoclonal Antibody 100 dtn
DNA/RNA Oxidative Damage EIA Standard 1 ea
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
589320-96well
DNA/RNA Oxidative Damage AChE Tracer 100 dtn
DNA/RNA Oxidative Damage EIA Monoclonal Antibody 100 dtn
DNA/RNA Oxidative Damage EIA Standard 1 ea
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
589320-480well
DNA/RNA Oxidative Damage AChE Tracer 500 dtn
DNA/RNA Oxidative Damage EIA Monoclonal Antibody 500 dtn
DNA/RNA Oxidative Damage EIA Standard 1 ea
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
589320-480SOLID
DNA/RNA Oxidative Damage AChE Tracer 500 dtn
DNA/RNA Oxidative Damage EIA Monoclonal Antibody 500 dtn
DNA/RNA Oxidative Damage EIA Standard ea
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Size Price Quantity Subtotal
96 solid wells $375.00 $0.00
96 strip wells $375.00 $0.00
480 solid wells $1,406.00 $0.00
480 strip wells $1,406.00 $0.00
Bulk Contact
Cart Total $0.00

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  • All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2014-04-19. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample DNA/RNA Oxidative Damage EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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FAQs

The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?

It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.

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Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?

The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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