Thiol Detection Assay Kit

Cayman Chemical Item Number 700340

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Description

Limit of detection: 15 nM · The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection Assay Kit provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Dickinson, D.A., and Forman, H.J. Cellular glutathione and thiols metabolism. Biochem Pharmacol 64 1019-1026 (2002).

Wendel, A., and Cikryt, P. The level and half-life of glutathione in human plasma. FEBS Lett 120 209-211 (1980).

Wlodek, L. Beneficial and harmful effects of thiols. Pol J Pharmacol 54 215-223 (2002).

Lash, L.H., and Jones, D.P. Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch Biochem Biophys 240 583-592 (1985).

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700340-96well
96-Well Cover Sheet
Half Volume 96-Well Solid Plate (black)
Thiol Assay Buffer (10X) 1 ea
Thiol Fluorometric Detector 1 ea
Thiol Glutathione Standard 1 ea
Thiol Cysteine Standard 1 ea
700340-480well
96-Well Cover Sheet
Half Volume 96-Well Solid Plate (black)
Thiol Assay Buffer (10X) 1 ea
Thiol Fluorometric Detector 5 × 1 ea
Thiol Glutathione Standard 1 ea
Thiol Cysteine Standard 1 ea
Size Price Quantity Subtotal
96 wells $178.00 $0.00
480 wells $668.00 $0.00
Bulk Contact
Cart Total $0.00

Pricing updated 2015-07-30. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Thiol Detection Assay Kit under the Cayman Challenge program.

Let us analyze your samples for you. See our contract research services.

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Batch-specific Information

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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