L-Lactate Assay Kit

Cayman Chemical Item Number 700510

L-Lactate Assay Kit

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Description

L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH). Monitoring lactate levels is, therefore, a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Cayman’s L-Lactate Assay provides a fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Formal Name
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Passarella, S., de Bari, L., Daniela, V., et al. Mitochondria and L-lactate metabolism. FEBS Lett 582 3569-3576 (2008).

Gladden, L.B. Lactate metabolism: A new paradigm for the third millenium. J Physiol 558(1) 5-30 (2004).

Bellomo, R. Bench-to-bedside review: lactate and the kidney. Crit Care 6(4) 322-326 (2002).

McLellan, A.C., Phillips, S.A., and Thornally, P.J. Flourimetric assay of D-lactate. Anal Biochem 206(1) 12-16 (1992).

Luft, F.C. Lactic acidosis update for critical care clinicians. J Am Soc Nephrol 12 S15-S19 (2001).

Robergs, R.A., Ghiasvand, F., and Parker, D. Biochemistry of exercise-induced metabolic acidosis. Am J Physiol Regul Integr Comp Physiol 287(3) R502-R516 (2004).

Du, W.B., Pan, X.P., and Li, L.J. Prognostic models for acute liver failure. Hepatobiliary Panceat Dis Int 9(2) 122-8 (2010).

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700510-96well
96-Well Cover Sheet
96-Well Solid Plate (black)
Orange Non-EIA Kit Box (96 Well)
Lactate Assay Buffer (10X) 10 mL
Lactate Cofactor Mixture 2 × 1 ea
Lactate Fluorescent Substrate 2 × 1 ea
L-Lactate Enzyme Mixture 2 × 1 ea
L-Lactate Standard 2 × 1 ea
Potassium Carbonate Assay Reagent 5 mL
MPA Assay Reagent 2 g
Size Price Quantity Subtotal
96 wells $325.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-23. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample L-Lactate Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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