Glutathione Reductase Assay Kit

Cayman Chemical Item Number 703202

GR Assay Kit

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Description

Glutathione reductase (GR, EC 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to glutathione (GSH).1 This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Cayman Chemical Glutathione Reductase Assay Kit measures GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. Since GR is present at rate limiting concentrations, the rate of decrease in the A340 is directly proportional to the GR activity in the sample. The Cayman GR Assay Kit is provided in a convenient 96 well plate format and can be used to measure GR activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

1. Carlberg, I., and Mannervik, B. Glutathione reductase. Methods Enzymol 113 484-490 (1985).

Synonyms
  • GR Assay Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Carlberg, I., and Mannervik, B. Glutathione reductase. Methods Enzymol 113 484-490 (1985).

703202-96WELL
96-Well Solid Plate (Colorimetric Assay)
GR Assay Buffer (10X) 1 ea
GR Sample Buffer (10X) 1 ea
GR Glutathione Reductase (control) 1 ea
GR GSSG 1 ea
GR NADPH 3 × 1 ea
Size Price Quantity Subtotal
96 wells $225.00 $0.00
Bulk Contact
Cart Total $0.00

Pricing updated 2015-07-30. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glutathione Reductase Assay Kit under the Cayman Challenge program.

Let us analyze your samples for you. See our contract research services.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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