TMB (hydrochloride hydrate)
Item № 70450
CAS № 207738-08-7
Purity ≥98%
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100 mg $29.00 $0.00
250 mg $43.00 $0.00
1 g $57.00 $0.00
5 g $214.00 $0.00

Pricing updated 2015-10-04. Prices are subject to change without notice.

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TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000).1 The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB.1,2 Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase.

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Technical Information
Formal Name 3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine, dihydrochloride, dihydrate
CAS Number 207738-08-7
Molecular Formula C16H20N2 • 2HCl [2H2O]
Formula Weight 349.3
Purity ≥98%
Formulation A crystalline solid
λmax 295 nm
SMILES CC1=C(N)C(C)=CC(C2=CC(C)=C(N)C(C)=C2)=C1.Cl.Cl.O.O
InChI Code 1S/C16H20N2.2ClH.2H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;;/h5-8H,17-18H2,1-4H3;2*1H;2*1H2

WARNING - This product is not for human or veterinary use.

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Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
Stability 1 year
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References & Background Reading
Product Description References

1. Josephy, P.D., Eling, T., and Mason, R.P. The horseradish peroxidase-catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Free radical and charge-transfer complex intermediates. J Biol Chem 257 3669-3675 (1982).

2. Marquez, L.A., and Dunford, H.B. Mechanism of the oxidation of 3,5,3',5'-tetramethylbenzidine by myeloperxidase determined by transient-and steady-state kinetics. Biochemistry 36 9349-9355 (1997).

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