TMPD (hydrochloride)

Cayman Chemical Item Number 70455

N,N,N',N'-Tetramethyl-p-Phenylenediamine; Wurster’s Reagent (CAS 637-01-4)

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TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases.1 TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm.2 Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels.3

1. Van der Ouderaa, F.J., Buytenhek, M., Nugteren, D.H., et al. Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands. Biochim Biophys Acta 487 315-331 (1977).

2. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

3. Butler, M.J., and Lachance, M.A. The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels. Anal Biochem 162 443-445 (1987).

  • N,N,N',N'-Tetramethyl-p-Phenylenediamine
  • Wurster’s Reagent
Formal Name N,​N,​N',​N'-​tetramethyl-​1,​4-​benzenediamine,​ dihydrochloride
CAS Number 637-01-4
Molecular Formula C10H16N2 • 2HCl
Formula Weight 237.2
Formulation A crystalline solid
Purity ≥95%
λmax 263 nm
Stability 2 years
Storage Room temperature
Shipping Room temperature in continental US; may vary elsewhere
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Size Price Quantity Subtotal
5 g $27.00 $0.00
10 g $51.00 $0.00
25 g $122.00 $0.00
50 g $216.00 $0.00
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Cart Total $0.00

Pricing updated 2015-07-06. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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TMB (hydrochloride hydrate)


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Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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Ann Arbor, Michigan 48108 USA


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