TMPD (hydrochloride)
Item № 70455
CAS № 637-01-4
Purity ≥95%
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5 g $27.00 $0.00
10 g $51.00 $0.00
25 g $122.00 $0.00
50 g $216.00 $0.00

Pricing updated 2015-11-30. Prices are subject to change without notice.

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  • N,N,N',N'-Tetramethyl-p-Phenylenediamine
  • Wurster’s Reagent

TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases.1 TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm.2 Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels.3

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Technical Information
Formal Name N,N,N',N'-tetramethyl-1,4-benzenediamine, dihydrochloride
CAS Number 637-01-4
  • N,N,N',N'-Tetramethyl-p-Phenylenediamine
  • Wurster’s Reagent
Molecular Formula C10H16N2 • 2HCl
Formula Weight 237.2
Purity ≥95%
Formulation A crystalline solid
λmax 263 nm
InChI Code 1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H

WARNING - This product is not for human or veterinary use.

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Storage Room temperature
Shipping Room temperature in continental US; may vary elsewhere
Stability 2 years
Product Downloads & Resources
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Download Product Insert 88 Kb PDF

Download Safety Data Sheet (SDS) 25 Kb PDF

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Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

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References & Background Reading
Product Description References

1. Van der Ouderaa, F.J., Buytenhek, M., Nugteren, D.H., et al. Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands. Biochim Biophys Acta 487 315-331 (1977).

2. Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

3. Butler, M.J., and Lachance, M.A. The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels. Anal Biochem 162 443-445 (1987).

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