Superoxide Dismutase Assay Kit • SOD Assay Kit || Cayman Chemical | Supplier
Superoxide Dismutase Assay Kit
Item № 706002
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $225.00 $0.00
480 wells $490.00 $0.00

Pricing updated 2015-08-29. Prices are subject to change without notice.

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Description
Synonyms
  • SOD Assay Kit

Significant amounts of superoxide dismutase (SOD) in cellular and extracellular environments are crucial for the prevention of diseases linked to oxidative stress. Mutations in SOD account for approximately 20% of familial amyotrophic lateral sclerosis (ALS) cases. SOD also appears to be important in the prevention of other neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington’s Diseases.1,2 The reaction catalyzed by SOD is extremely fast, having a turnover of 2 x 106 M−1sec−1 and the presence of sufficient amounts of the enzyme in cells and tissues typically keeps the concentration of superoxide very low.3 Quantification of SOD activity is therefore essential in order to fully characterize the antioxidant capabilities of a biological system. The Cayman Chemical SOD Assay kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96 well format. A key feature of the kit is the inclusion of a quality-controlled SOD standard. The standard curve generated using this enzyme provides a means to accurately quantify the activity of all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Each kit contains sufficient reagents to assay 41 samples in duplicate and includes assay buffer, sample buffer, radical detector, SOD (standard), xanthine oxidase, a 96 well plate, and complete instructions.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Download Kit Booklet Download Safety Data Sheet (SDS)

Technical Information
Synonyms
  • SOD Assay Kit

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability 1 year
Product Downloads & Resources
Product Downloads

Download Kit Booklet 452 Kb PDF

Download Safety Data Sheet (SDS) 89 Kb PDF

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

Inserts
QC Sheets
Certificates of Analysis
GCMS Data
References & Background Reading
Product Description References

1. Maier, C.M., and Chan, P.H. Role of superoxide dismutases in oxidative damage and neurodegenerative disorders. Neuroscientist 8(4) 323-334 (2002).

2. Liu, D. The roles of free radicals in amyotrophic lateral sclerosis. J Mol Neurosci 7 159-167 (1996).

3. Malström, B., Andreasson, L., and Reinhammer, B. XIIB. 533 (1975).

Background Reading

Liu, D. The roles of free radicals in amyotrophic lateral sclerosis. J Mol Neurosci 7 159-167 (1996).

Malström, B., Andreasson, L., and Reinhammer, B. XIIB. 533 (1975).

Maier, C.M., and Chan, P.H. Role of superoxide dismutases in oxidative damage and neurodegenerative disorders. Neuroscientist 8(4) 323-334 (2002).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Superoxide Dismutase Assay Kit

View all analysis workbooks

View the pre-configured analysis tool for Superoxide Dismutase Assay Kit on MyAssays.com

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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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Ann Arbor, Michigan 48108 USA

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