Superoxide Dismutase Assay Kit

Cayman Chemical Item Number 706002

SOD Assay Kit

Superoxide Dismutase Assay Kit

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Description

Significant amounts of superoxide dismutase (SOD) in cellular and extracellular environments are crucial for the prevention of diseases linked to oxidative stress. Mutations in SOD account for approximately 20% of familial amyotrophic lateral sclerosis (ALS) cases. SOD also appears to be important in the prevention of other neurodegenerative disorders such as Alzheimer’s, Parkinson’s, and Huntington’s Diseases.1,2 The reaction catalyzed by SOD is extremely fast, having a turnover of 2 x 106 M−1sec−1 and the presence of sufficient amounts of the enzyme in cells and tissues typically keeps the concentration of superoxide very low.3 Quantification of SOD activity is therefore essential in order to fully characterize the antioxidant capabilities of a biological system. The Cayman Chemical SOD Assay kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates, and cell lysates. SOD activity is assessed by measuring the dismutation of superoxide radicals generated by xanthine oxidase and hypoxanthine in a convenient 96 well format. A key feature of the kit is the inclusion of a quality-controlled SOD standard. The standard curve generated using this enzyme provides a means to accurately quantify the activity of all three types of SOD (Cu/Zn-, Mn-, and Fe-SOD). Each kit contains sufficient reagents to assay 41 samples in duplicate and includes assay buffer, sample buffer, radical detector, SOD (standard), xanthine oxidase, a 96 well plate, and complete instructions.

1 Maier, C.M., and Chan, P.H. Role of superoxide dismutases in oxidative damage and neurodegenerative disorders. Neuroscientist 8(4) 323-334 (2002).

2 Liu, D. The roles of free radicals in amyotrophic lateral sclerosis. J Mol Neurosci 7 159-167 (1996).

3 Malström, B., Andreasson, L., and Reinhammer, B. XIIB. 533 (1975).

Synonyms
  • SOD Assay Kit
Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Maier, C.M., and Chan, P.H. Role of superoxide dismutases in oxidative damage and neurodegenerative disorders. Neuroscientist 8(4) 323-334 (2002).

Malström, B., Andreasson, L., and Reinhammer, B. XIIB. 533 (1975).

Liu, D. The roles of free radicals in amyotrophic lateral sclerosis. J Mol Neurosci 7 159-167 (1996).

706002-96WELL
96-Well Solid Plate (Colorimetric Assay)
SOD Assay Buffer (10X) 1 ea
SOD Assay Sample Buffer (10X) 1 ea
SOD Assay Radical Detector 1 ea
SOD Standard 1 ea
SOD Assay Xanthine Oxidase 3 × 1 ea
706002-480well
96-Well Solid Plate (Colorimetric Assay)
SOD Assay Buffer (10X) 5 ea
SOD Assay Sample Buffer (10X) 5 ea
SOD Assay Radical Detector 2 × 1 ea
SOD Standard 2 × 1 ea
SOD Assay Xanthine Oxidase 6 × 1 ea
Size Price Quantity Subtotal
96 wells $225.00 $0.00
480 wells $490.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-19. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Superoxide Dismutase Assay Kit under the Cayman Challenge program.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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