Antioxidant Assay Kit

Cayman Chemical Item Number 709001

Antioxidant Assay Kit

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Description

The antioxidant system of living organisms includes enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules such as albumin, ceruloplasmin, and ferritin; and an array of small molecules, including ascorbic acid, α-tocopherol, β-carotene, reduced glutathione, uric acid, and bilirubin. The sum of endogenous and food-derived antioxidants represents the total antioxidant activity of the extracellular fluid. Cooperation of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals, than any single compound alone. Thus, the overall antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids. The Cayman Chemical Antioxidant Assay Kit measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2'-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS® · + by metmyoglobin.1,2,3,4 The capacity of the antioxidants in the sample to prevent ABTS oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as molar Trolox equivalents. Each kit contains assay buffer (10X), chromogen, metmyoglobin, trolox, hydrogen peroxide, a 96 well plate, plate cover, and complete instructions.

1 Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84 407-412 (1993).

2 Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS•+ radical cation assay . Free Radic Res 26 195-199 (1997).

3 Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity. Biochem Soc Trans 21 95S (1993).

4 Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids. Methods Enzymol 234(24) 279-293 (1994).

Formal Name
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity. Biochem Soc Trans 21 95S (1993).

Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids. Methods Enzymol 234(24) 279-293 (1994).

Miller, N.J., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84 407-412 (1993).

Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS•+ radical cation assay . Free Radic Res 26 195-199 (1997).

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709001-96WELL
Antioxidant Assay Buffer (10X) 1 ea
Antioxidant Assay Chromogen 3 × 1 ea
Antioxidant Assay Metmyoglobin 2 × 1 ea
Antioxidant Assay Trolox 3 × 1 ea
Antioxidant Assay Hydrogen Peroxide 1 ea
96-Well Solid Plate (Colorimetric Assay)
Size Price Quantity Subtotal
96 wells $230.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-20. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Antioxidant Assay Kit under the Cayman Challenge program.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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