Novel Photostable Metal Ion Complex Fluorescent Cell Imaging Reagents | Cayman Chemical
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Novel, Photostable, Metal Ion Complex Fluorescent Cell Imaging Reagents

2018-10-01

As far back as the 1950s, metal ion complexes have been used as imaging agents for detailed detection of dynamic intracellular processes, particularly in relation to disease pathogenesis. These complexes can selectively accumulate in organelles of interest through the ability of chemists to alter their chelating ligands. Recently, a set of these imaging agents, known as ReZolve and IraZolve dyes became commercially available and are transforming the way scientists visualize energy storage, signaling, cell metabolism, and major structural components of cellular membranes.

How Do They Work?

ReZolve and IraZolve dyes are derived from low-spin transition metal complexes (e.g., Re(I) tricarbonyl tetrazolo complexes and cyclometalated Ir(III) complexes). Emission from the low-spin metal ion complex results from charge transfer between the metal center and highly conjugated ligands. The charge transfer between metal and ligand produce long-lived fluorescent emission. These complexes are also color-tunable in that the emission of the charge transfer can be altered by incorporation of electron-withdrawing and electron-donating substituents within the metal-bound ligands that modulate the energy gap between the highest occupied and lowest unoccupied molecular orbitals.

Unique Advantages

These unique metal ion complex dyes exhibit high quantum yields, long-lived (millisecond) visible emission, are resistant to photobleaching, and have been developed to allow for live cell imaging. The clear advantage of this technology over small fluorescent molecules or fluorescently labelled antibodies is that it overcomes the limitations of photobleaching, short (nanosecond) emissive lifetimes, and the need for cell fixation.

Overview of Metal Ion Complex Dyes

Properties ReZolve-L1™ IraZolve-L1™ ReZolve-ER™ IraZolve-Mito™ ReZolve-Alkyne™
LocalizationPolar Lipids and Lipid-Rich CompartmentsLipid Droplets and Endoplasmic ReticulumEndoplasmic ReticulumMitochondriaUser-Defined Luminescent Tag
ColorYellowRedOrangeRedOrange
Resistance to photobleachingHighHighHighHighHigh
CytotoxicityLowLow
LowLowLow
Ex/Em (nm)UV or 405/550405/600UV or 405/570405/600UV or 405/570
Live Cells and Tissue CompatibleYesYesYesYesYes
Fixed Cells and Tissues CompatibleYesYesYesFixed Tissue Only, Not Fixed CellsYes
SolubilityDMSODMSODMSODMSODMSO
Fluorescence and Multiphoton Microscopy CompatibleYesYesYesYesYes
Raman and Infrared Spectroscopy CompatibleYesNoYesNoYes
Storage and TransportRoom TempRoom TempRoom TempRoom Temp (4°C after reconstitution)Room Temp
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Lipid Staining

Both ReZolve-L1™ and IraZolve-L1™ can be used to detect lipid droplets in lipid storage cells (Figure 1). ReZolve-L1™ has also been shown to be unique for labelling polar lipids and lipid-rich compartments. This includes cholesterol, sphingolipids, and phospholipids. These dyes have been used for effective monitoring of cellular lipid content and localization, which is particularly useful in the investigation of metabolic disease. Their spectral qualities are compatible with other commercially available fluorophores for multiplexed imaging.

Figure 1 lipid droplets in adipocytes.png

Figure 1. ReZolve-L1™ detected in lipid droplets in adipocytes.

Endoplasmic Reticulum Staining

ReZolve-ER™ has rapid cell uptake and can be used for staining of the endoplasmic reticulum (ER), which allows observation of dynamic changes that occur in the ER (Figure 2). The use of this dye does not require wash steps, which decreases preparation time. This makes it ideal for time-dependent experiments or drug treatment assays where multiple wash steps can interrupt work flow. The stain is reversible and can be washed from cells after imaging. While IraZolve-L1™ localizes in lipid droplets, it can also accumulate in the ER.

Figure 2 live prostate cells.png Figure 2 paraformaldehyde fixed prostate cells.png

Figure 2. ReZolve-ER™ in live and 4% paraformaldehyde-fixed prostate cells.

Mitochondria Staining

IraZolve-Mito™ is uniquely suitable for the detection of mitochondria in fixed and frozen tissue samples, as well as live cells (Figure 3). It has rapid cell uptake and allows for quick and easy detection of mitochondria in samples, without the need for antibody staining. Because it is amenable to long-term storage, IraZolve-Mito™ is ideal for animal trials where multiple samples are collected and stored for later analysis.

Figure 3 live H9C2 cells.png

Figure 3. IraZolve-Mito™ on live H9C2 cells and images at 20x.

Click Chemistry Probe

ReZolve-Alkyne™ can be used to track a compound of interest in cells or tissues by tagging azide functionalized molecules (Figure 4). This luminescent alkyne has a large Stokes shift, long emission lifetime, and is resistant to photobleaching. It can be conjugated to a range of chemicals and requires only simple or even no workup or purification of the product. Resulting products can be detected by fluorescence, infrared spectroscopy, Raman spectroscopy, X-ray fluorescence and inductively coupled plasma mass spectrometry, making it an ideal tool for both chemists and biologists.

Figure 4 copper facilitated click reaction.png

Figure 4. ReZolve-Alkyne™ is suitable for copper-facilitated click reaction.

Recommendations to Achieve Best Results

StepExpert AdviceRecommendation
Fluorophore concentration/dilutionThere is a tendency to think more is better, but ReZolve products have an ideal concentration range. Using the dyes at too high a concentration can cause the dyes to precipitate, which then will not enter the cells. All ReZolve dyes should be used at a final concentration of less than 50 µM, and for most, 10-20 µM is recommended. Verify the protocol and recommended concentration for each product.
Sample preparationAll ReZolve dyes work well in live cells. However, there are some limitations in fixed cell preparations.
For example:
  • Harsh fixation can remove lipids, which many of the dyes require for localization
  • Ample permeabilization removes lipids. Paraffin-embedded samples are generally not compatible with these dyes
Fixation at 2-4% PFA for 10-20 minutes is recommended to retain lipids, which enable localization.
Incubation timeProtocols for ReZolve dyes are only guidelines. Incubation times may need to be adjusted depending on the sample.Longer or shorter incubation times may work better for your particular sample.
Mounting media or sample storageGlycerol is a suitable mounting media but can dull the signal and extract the dye if the sample is stored for too long before imaging.Water-based mounting media is recommended with ReZolve dyes. For best results, image the sample the same day as staining.
ImagingReZolve protocols provide a general recommendation for imaging. However, each microscopy system is different. Samples can vary greatly, and in some cases, emission/detection levels may seem faint.Imaging can be improved by using lower capture speeds, or by leaving the detection open for longer to improve signal collection. The capture speed may be difficult to adjust when working with live samples, which can move during image collection.
Imaging settingsReZolve dyes excite with a UV or 405 nm light source, unlike most blue-excited dyes, which have emission of 550-570 nm or 570-600 nm. Some older systems require filters for the excitation source, as well as the emitted light. If the filters are incorrect in epifluorescence microscopy, the excitation light can be blocked altogether.Verify the emission filter is set appropriately for imaging FITC or Texas Red dyes. Verify filters (excitation source and emission light filters) are correct when using older systems. Confocal microscopy does not have this issue.
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Go to www.caymanchem.com/ReZolve to learn more about these cell imaging reagents


Additional Reading

Gillam, T.A., Sweetman, M.J., Bader, C.A., et al. Bright lights down under: Metal ion complexes turning the spotlight on metabolic processes at the cellular level. Coord. Chem. Rev. 375, 234-255 (2018).


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