Acylated Ghrelin (human) Express EIA Kit | Cayman Chemical
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Acylated Ghrelin (human) Express EIA Kit

Item № 10006306
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $504.00 0.00
     384 wells $1,235.00 0.00

Pricing updated 2018-08-16. Prices are subject to change without notice.

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Description
Features
  • Measure acetylated ghrelin in human plasma samples
  • 96 well kit: Assay 24 samples in triplicate or 36 samples in duplicate
  • 384 well kit is supplied as 1 x 384 well plate for HTS applications
  • Incubation: 3 hours
  • Sampling tubes with PHMB available to store, preserve, and protect samples from protein degradation (see Item No. 10949)
Synonyms
  • Ghrelin (human acylated) ELISA

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. The peptide consists of 28 amino acids, with an octanoylation of the serine-3 residue, which is necessary for biological activity. Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. This EIA kit specifically measures the acylated form of ghrelin. This EIA is based on a double-antibody sandwich technique. The wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab' conjugate which recognizes the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin. Each kit contains ghrelin (acylated) AChE-Fab' conjugate, human acylated ghrelin standard, human acylated ghrelin quality control, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions. [Bertin Catalog No. A05106.384 wells]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Ghrelin (human acylated) ELISA
Specificity
  • (100%) Human Acylated Ghrelin
  • (118%) Rat Acylated Ghrelin
  • (<0.001%) Human CRF
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Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 2 years
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support
Analysis Tools

View our analysis workbook for Acylated Ghrelin (human) Express EIA Kit

View all analysis workbooks

FAQ

In the Ghrelin (human acylated) EIA Kit when using plasma samples, is it possible to use AEBSF rather than PHMBA?

It appears that the AEBSF interferes with the enzyme activity of the acetylcholine esterase on the tracer in the Ghrelin (human acylated) EIA Kit. There is a way around this. It is possible to perform the assay in a two step process. The first step is incubating samples and standards with the immobilized antibody on the plate. The second step involves washing the wells to remove the protease inhibitors then incubation with the detection antibody/AChE conjugate.

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You may be eligible to receive a free sample of Acylated Ghrelin (human) Express EIA Kit under the Cayman Challenge program.

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Ann Arbor, Michigan 48108 USA

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