Pricing updated 2018-05-27. Prices are subject to change without notice.
Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that control energy homeostasis. The peptide consists of 28 amino acids, with an octanoylation of the serine-
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In the Ghrelin (human acylated) EIA Kit when using plasma samples, is it possible to use AEBSF rather than PHMBA?
It appears that the AEBSF interferes with the enzyme activity of the acetylcholine esterase on the tracer in the Ghrelin (human acylated) EIA Kit. There is a way around this. It is possible to perform the assay in a two step process. The first step is incubating samples and standards with the immobilized antibody on the plate. The second step involves washing the wells to remove the protease inhibitors then incubation with the detection antibody/AChE conjugate.