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SREBP-2 Polyclonal Antibody

Item № 10007663
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     1 ea $279.00 0.00

Pricing updated 2019-07-17. Prices are subject to change without notice.

Description
Synonyms
  • SREBF2
  • Sterol Regulatory Element-binding Protein-2
  • Sterol Regulatory Element-binding Transcription Factor 2

SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism.1 SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.2 SREBP-2 is ubiquitously detected in various tissues.3 Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.4 Cayman’s SREBP-2 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, HepG2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.5

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Technical Information
Synonyms
  • SREBF2
  • Sterol Regulatory Element-binding Protein-2
  • Sterol Regulatory Element-binding Transcription Factor 2
Immunogen
Synthetic peptide from an internal region of human SREBP-2
Formulation
500 μl of peptide-affinity purified antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
ICC, IHC, and WB
Species Reactivity
  • (+) Human
  • (+) Mouse
  • (+) Rat
UniProt Accession №
Q12772
Origin
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

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Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor Cell 89, 331-340 (1997).

2. Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies The Journal of Biological Chemisty 273(10), 5785-5793 (1998).

3. Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis Proceedings of the National Academy of Sciences of the United States of America 101(19), 7281-7286 (2004).

4. Smith, L.H., Petrie, M.S., Morrow, J.D., et al. The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells Journal of Lipid Research 46, 862-871 (2005).

5. Hua, X., Sakai, J., Ho, Y.K., et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection The Journal of Biological Chemisty 270(49), 29422-29427 (1995).

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