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TBARS Assay Kit

Item № 10009055
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     96 wells $185.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure TBARS in plasma, serum, urine, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Assay range: 0.0625-50 µM
  • Plate-based colorimetric measurement (530-540 nm) or fluorometric measurement (ex 530 nm, em 550 nm)
  • Thiobarbituric Acid Reactive Substances

Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from PUFAs results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these 'Thiobarbituric Acid Reactive Substances' (TBARS) is a well-established method for screening and monitoring lipid peroxidation.1,2 Modifications of the TBARS assay has been used to evaluate a wide range of samples that include human and animal tissues and fluids, drugs and foods. Even though there remains a controversy cited in literature regarding the specificity of TBARS toward compounds other than MDA, it still remains the most widely employed assay used to determine lipid peroxidation. Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Thiobarbituric Acid Reactive Substances

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma Methods in Molecular Biology 108, 101-106 (1998).

2. Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory Free Radicals in Diagnostic Medicine 366, 43-58 (1994).

Product Citations

Wang, W., Green, M., Choi, J.E., et al. CD8+ T cells regulate tumour ferroptosis during cancer immunotherapy Nature 569(7755), 270-274 (2019).

Wen, J.J., Yin, Y.W., and Garg, N.J. PARP1 depletion improves mitochondrial and heart function in Chagas disease: Effects on POLG dependent mtDNA maintenance PLoS Pathog. 14(5), e1007065 (2018).

Wen, J.J., and Garg, N.J. Manganese superoxide dismutase deficiency exacerbates the mitochondrial ROS production and oxidative damage in Chagas disease PLoS Negl. Trop. Dis. 12(7), e0006687 (2018).

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