Pricing updated 2018-02-18. Prices are subject to change without notice.
Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.1,2 The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitro or in vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5x108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry.
Warning - this product is not for human or veterinary use.
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.
1. Parish, C.R.,Glidden, M.H.,Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current Protocols in Immunology Supp. 84, 4.9.1-4.9.13 (2009).
2. Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. Journal of Immunological Methods 243, 147-154 (2000).