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CFSE Cell Division Assay Kit

Item № 10009853
product image
     100 tests $145.00 0.00

Pricing updated 2019-06-15. Prices are subject to change without notice.

  • Flow cytometric analysis of cell proliferation
  • Cell division results in a halving of the fluorescent signal; up to eight divisions can be monitored
  • Compatible with staining for cell-surface and intracellular markers
  • 5-Carboxyfluorescein NHS ester
  • 5-Carboxyfluorescein-N-hydroxysuccinimide ester
  • 5-Carboxyfluorescein N-Succinimidyl ester
  • 5-FAM N-succinimidyl ester​

Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.1,2 The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitro or in vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5x108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • 5-Carboxyfluorescein NHS ester
  • 5-Carboxyfluorescein-N-hydroxysuccinimide ester
  • 5-Carboxyfluorescein N-Succinimidyl ester
  • 5-FAM N-succinimidyl ester​

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Parish, C.R., Glidden, M.H., Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation Curr. Protoc. Immunol. Supp. 84, 4.9.1-4.9.13 (2009).

2. Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution J. Immunol. Methods 243(1-2), 147-154 (2000).

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