XTT Cell Proliferation Assay Kit

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.¹ Cayman’s XTT cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.² Reduction of XTT produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. This kit will allow investigators to screen drug candidates involved in cell cycle regulation.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

WARNING This product is not for human or veterinary use.

1. Francoeur, A.M., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3, 19-25 (1996).

2. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Ann. Rev. 11, 127-152 (2005).