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XTT Cell Proliferation Assay Kit

Item № 10010200
product image
     96 wells $50.00 0.00
     480 wells $98.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • A colorimetric, non-radioactive assay for assessing cell viability and proliferation
  • Tetrazolium salt, XTT, is reduced to a soluble purple formazan by trans-plasma membrane electron transport from NADH via an electron mediator
  • Detect 500-100,000 cells per well
  • Rapid assay; get results in about 4 hours

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.1 Cayman’s XTT cell proliferation assay kit provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.2 Reduction of XTT produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. This kit will allow investigators to screen drug candidates involved in cell cycle regulation.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Francoeur, A.M., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1 Biochemica 3, 19-25 (1996).

2. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction Biotechnol. Ann. Rev. 11, 127-152 (2005).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640