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SREBP-1 Transcription Factor Assay Kit

Item № 10010854
product image
     96 wells $425.00 0.00

Pricing updated 2019-07-23. Prices are subject to change without notice.

  • A sensitive, non-radioactive method of detecting SREBP-1 from whole cell lysates
  • 96-well plate format replaces EMSAs
  • Capture the transcription factor using a specific dsDNA sequence bound to the plate
  • Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
  • Nuclear Extraction Kit (Item No. 10009277) available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates
  • Sterol Regulatory Element-Binding Protein-1 Assay Kit

Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman’s SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Sterol Regulatory Element-Binding Protein-1 Assay Kit
  • Animal/Bovine
  • Animal/Goat
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Dry ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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Ann Arbor, Michigan 48108 USA

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