- A sensitive, non-radioactive method of detecting SREBP-1 from whole cell lysates
- 96-well plate format replaces EMSAs
- Capture the transcription factor using a specific dsDNA sequence bound to the plate
- Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
- Nuclear Extraction Kit (Item No. 10009277) available to aid in the isolation of nuclear and cytoplasmic fractions from cell lysates or tissue homogenates
- Sterol Regulatory Element-Binding Protein-1 Assay Kit
Three known isoforms of SREBP (sterol regulatory element binding protein) transcription factors have been characterized: SREBP-1a, SREBP-1c, and SREBP-2. SREBP-1c acts primarily to activate genes required for fatty acid synthesis, such as acetyl CoA carboxylase, fatty acid synthase, and long chain fatty acid elongase. In addition, SREBP-1c may also contribute to the regulation of glucose uptake and synthesis through induction of glucokinase. SREBP-1c has important clinical implications in the treatment of many diseases including obesity, diabetes mellitus, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Cayman’s SREBP-1 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity. SREBP-1 contained in nuclear extracts and whole cell lysates binds to a dsDNA SREBP response element immobilized to the wells of a 96-well plate. SREBP-1 is detected by addition of a specific primary antibody directed against SREBP-1. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.