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Item № 13013
CAS № 856925-71-8
Purity ≥98%
product image
                (CAS 856925-71-8)
     1 mg $45.00 0.00
     5 mg $169.00 0.00
     10 mg $315.00 0.00
     25 mg $619.00 0.00

Pricing updated 2019-06-15. Prices are subject to change without notice.

  • (S)-Blebbistatin

(–)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases and the active enantiomer of (+)-blebbistatin (Item No. 13165).1,2 It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM).3 Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.4,5 (S)-nitro-Blebbistatin (Item No. 13186) is a more stable form of (–)-blebbistatin.6 The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure. (S)-nitro-Blebbistatin has the same stereochemistry as the active (–)-blebbistatin enantiomer.

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Technical Information
Formal Name
CAS Number
  • (S)-Blebbistatin
Molecular Formula
Formula Weight
A crystalline solid
236, 270, 298, 418 nm
InChI Code
InChI Key

Warning - this product is not for human or veterinary use.

Shipping & Storage
Room temperature in continental US; may vary elsewhere
≥ 2 years
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Additional Information

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References & Product Citations
Product Description References

1. Straight, A.F., Cheung, A., Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor Science 299(5613), 1743-1747 (2003).

2. Kovács, M., Tóth, J., Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II J. Biol. Chem. 279(34), 35557-35563 (2004).

3. Limouze, J., Straight, A.F., Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II J. Muscle Res. Cell Motil. 25(4-5), 337-341 (2004).

4. Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light Biochem. Biophys. Res. Commun. 320(3), 1020-1025 (2004).

5. Sakamoto, T., Limouze, J., Combs, C.A., et al. Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light Biochemistry 44(2), 584-588 (2005).

6. Lucas-Lopez, C., Patterson, S., Blum, T., et al. Absolute stereochemical assignment and fluorescence tuning of the small molecule tool, (−)-blebbistatin European J. Org. Chem. 2005(9), 1736-1740 (2005).

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