An activator of KCa channels
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1-EBIO

Item No. 14575

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1-ethyl-1,3-dihydro-2H-benzimidazol-2-one
CAS Number
10045-45-1
Synonyms
  • 1-Ethylbenzimidazolinone
Molecular Formula
C9H10N2O
Formula Weight
162.2
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 100 mg/mlDMSO: 100 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/mlEthanol: 30 mg/ml
λmax
229, 285 nm
SMILES
O=C(N1CC)NC2=C1C=CC=C2
InChi Code
InChI=1S/C9H10N2O/c1-2-11-8-6-4-3-5-7(8)10-9(11)12/h3-6H,2H2,1H3,(H,10,12)
InChi Key
CXUCKELNYMZTRT-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $36

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    10 mg

    $55

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    50 mg

    $213

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    100 mg

    $389

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    Product Description

    1-EBIO is an activator of Ca2+-sensitive K+ channels (EC50 = 490 µM in T84 human carcinoma cells).1 This action is sensitive to the neurotoxin charybdotoxin (Ki = 3.5 nM). In mouse colonic epithelia, 1-EBIO also activates cAMP-sensitive K+ channels, a response this is inhibited by 293B.2 In this type of epithelium, but not mouse nasal epithelia, 1-EBIO activates both types of channels, resulting in large Cl- secretory currents.3 It also activates the human intermediate conductance Ca2+-activated K+ channel (EC50 = 74 µM).4 1-EBIO is currently used to study the role of these types of K+ channels in diverse physiological functions.5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Devor, D.C., Singh, A.K., Frizzell, R.A., et al. Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca(2+)-dependent K+ channel. Am. J. Physiol. 271(5 Pt 1), L775-L784 (1996).

    2. Cuthbert, A.W., Hickman, M.E., Thorn, P., et al. Activation of Ca2+- and cAMP-sensitive K+ channels in murine colonic epithelia by 1-ethyl-2-benzimidazolone. Am. J. Physiol. 277(1), C111-C120 (1999).

    3. MacVinish, L.J., Hickman, M.E., Mufti, D.A., et al. Importance of basolateral K+ conductance in maintaining Cl- secretion in murine nasal and colonic epithelia. J. Physiol. 510(1), 237-247 (1998).

    4. Jensen, B.S., Strobæk, D., Christophersen, P., et al. Characterization of the cloned human intermediate-conductance Ca2+-activated K+ channel. Am. J. Physiol. 275(3), C848-C856 (1998).

    5. Gao, Z., Todorov, B., Barrett, C.F., et al. Cerebellar ataxia by enhanced Cav2.1 currents is alleviated by Ca2+-dependent K+-channel activators in Cacna1aS218L mutant mice. J. Neurosci. 32(44), 15533-15546 (2012).

    6. Maiz, J., Karakossian, M.H., Pakaprot, N., et al. Prolonging the postcomplex spike pause speeds eyeblink conditioning. Proc. Natl. Acad. Sci. USA 109(41), 16726-16730 (2012).