PPARδ (human) Reporter Assay Kit

Item № 15731
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $715.00 0.00
3 x 32 wells $735.00 0.00
384 wells $1,925.00 0.00

Pricing updated 2018-02-25. Prices are subject to change without notice.

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Description
Features
  • Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against human PPARδ
  • Robust technology provides sustained response for consistent and reproducible data acquistion
  • Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate
Synonyms
  • NR1C2
  • ​Peroxisome Proliferator-activated Receptor δ
  • PPARD
  • hPPARδ​

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human peroxisome proliferator-activated receptor delta (NR1C2), a ligand-dependent transcription factor commonly referred to as PPARD or PPARδ. INDIGO’s reporter cells include the luciferase reporter gene functionally linked to a PPARδ-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in PPARδ activity. The principal application of this reporter assay system is in the screening of test samples to quantify any functional activity, either agonist or antagonist, that they may exert against human PPARδ. PPARδ reporter cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the preincubation of reporter cells prior to assay setup. INDIGO Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to PPARδ reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a reference agonist, luciferase detection reagent, and a cell culture-ready assay plate. [INDIGO Catalog No. IB0015]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • NR1C2
  • ​Peroxisome Proliferator-activated Receptor δ
  • PPARD
  • hPPARδ​

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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