ERα (human) Reporter Assay Kit | Cayman Chemical
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ERα (human) Reporter Assay Kit

Item № 15737
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $715.00 0.00
     3 x 32 wells $735.00 0.00
     384 wells $1,925.00 0.00

Pricing updated 2018-08-14. Prices are subject to change without notice.

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Description
Features
  • Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against human ERα
  • Robust technology provides sustained response for consistent and reproducible data acquistion
  • Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate
Synonyms
  • ​Estrogen Receptor α
  • ​NR3A1

This nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 1 (NR3A1), a ligand-dependent transcription factor commonly referred to as ERα. INDIGO’s reporter cells include the luciferase reporter gene functionally linked to an ERα-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in ERα activity. Luciferase gene expression occurs after ligand-bound ERα undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the target gene. Unlike some other cell-based assay strategies, the readout from INDIGO’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. ERα reporter cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the pre-incubation of reporter cells prior to assay setup. The principal application of this assay product is in the screening of test samples to quantify functional activities, either agonist or antagonist, that they may exert against the human estrogen receptor. It is an all-inclusive assay system that includes, in addition to ERα reporter cells, two optimized media for use during cell culture and in diluting the test samples, a reference agonist, luciferase detection reagent, a cell culture-ready assay plate, and a detailed protocol. [INDIGO Catalog No. IB0040]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • ​Estrogen Receptor α
  • ​NR3A1

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
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Download Kit Booklet

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Additional Information

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