CAR3 (human) Reporter Assay Kit | Cayman Chemical
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CAR3 (human) Reporter Assay Kit

Item № 15747
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $715.00 0.00
     3 x 32 wells $735.00 0.00
     384 wells $1,925.00 0.00

Pricing updated 2018-08-15. Prices are subject to change without notice.

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Description
Features
  • Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against CAR3
  • Robust technology provides sustained response for consistent and reproducible data acquistion
  • Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate
Synonyms
  • Constitutive Androstane Receptor 3
  • Constitutive Androstane Receptor, Isoform 3
  • NR1I3

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human constitutive androstane receptor, isoform 3 (NR1I3), a ligand-dependent transcription factor commonly referred to as CAR3. These reporter cells utilize a modified version of human CAR3 in which the native N-terminal DNA binding domain (DBD) has been replaced with that of the GAL4-DBD. The human CAR3 ligand binding domain (LBD) is unaltered and fully functional. The reporter cells also incorporate a luciferase cDNA functionally linked to the GAL4-upstream activation sequence (UAS). Thus, quantifying expressed luciferase activity provides a sensitive surrogate measure of changes in CAR3 activity resulting from direct interaction between a treatment compound and the nuclear receptor. Because this assay system expresses the [GAL4-DBD + hCAR3 LBD] hybrid receptor, the activity of modulators that act through indirect mechanisms (such as those that alter the phosphorylation status of the native N-terminal amino acid sequence of the CARs) may be dampened or go undetected. Contrary to its name, human CAR3 is not constitutively active, rather, it exhibits ligand-dependent activation. The primary application of this reporter assay system is in the screening of test compounds to quantify any functional activity, either agonist or antagonist, that they may exert on human CAR3. Reporter cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryopreservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. INDIGO Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to CAR3 reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a positive-control agonist, luciferase detection reagent, and a cell culture-ready assay plate. [INDIGO Catalog No. IB0090]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Constitutive Androstane Receptor 3
  • Constitutive Androstane Receptor, Isoform 3
  • NR1I3

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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