CAR1 (human) Reporter Assay Kit | Cayman Chemical
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CAR1 (human) Reporter Assay Kit

Item № 15748
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $715.00 0.00
     3 x 32 wells $735.00 0.00

Pricing updated 2018-09-19. Prices are subject to change without notice.

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Description
Features
  • Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against CAR1
  • Robust technology provides sustained response for consistent and reproducible data acquistion
  • Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate
Synonyms
  • Constitutive Androstane Receptor 1
  • Constitutive Androstane Receptor, Isoform 1
  • NR1I3

This assay utilizes proprietary non-human mammalian cells that express isoform 1 of human constitutive androstane receptor (NR1I3), a ligand-dependent transcription factor referred to as CAR1. These reporter cells utilize a modified version of human CAR1 in which the N-terminal DNA binding domain (DBD) has been replaced with the GAL4-DBD. The human CAR1 ligand binding domain (LBD) is unaltered and fully functional. The reporter cells also incorporate a luciferase cDNA functionally linked to the GAL4-upstream activation sequence (UAS). Thus, quantifying expressed luciferase activity provides a sensitive surrogate measure of changes in CAR1 activity resulting from direct interaction between a treatment compound and the nuclear receptor. Because this assay system expresses a GAL4-DBD + hCAR1 LBD hybrid receptor, the bioactivity of activators that act through indirect mechanisms (such as those that alter the phosphorylation status of the native N-terminal amino acid sequence of CAR1) may be dampened or go undetected. Human CAR1 exhibits constitutive activity, but its activity can be further regulated through direct ligand interactions. The primary application of this reporter assay system is in the screening of test samples to quantify any functional inverse-agonist activity that they may exert on human CAR1. Reporter cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. INDIGO Bioscience’s nuclear receptor reporter assays are all-inclusive cell-based assay systems. In addition to CAR1 reporter cells, this kit provides two optimized media for use during cell culture and in diluting the user’s test samples, a positive-control inverse-agonist, luciferase detection reagent, and a cell culture-ready assay plate. [INDIGO Catalog No. IB0091]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Constitutive Androstane Receptor 1
  • Constitutive Androstane Receptor, Isoform 1
  • NR1I3

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
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Download Kit Booklet

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Additional Information

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