Human NF-κB Reporter Assay System | Cayman Chemical
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Human NF-κB Reporter Assay System

Item № 15953
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 wells $715.00 0.00
     3 x 32 wells $735.00 0.00
     384 wells $1,925.00 0.00

Pricing updated 2018-09-19. Prices are subject to change without notice.

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Description

This assay system utilizes HEK293t cells that express NF-κB (nuclear factor kappa-lightchain enhancer of activated B cells) and contain the luciferase reporter gene functionally linked to upstream NF-κB genetic response elements. Thus, quantifying changes in luciferase expression provides a sensitive surrogate measure of changes in the level of NF-κB activation. NF-κB is a signal transduction dependent transcription factor. This NF-κB reporter cell line is validated to provide a robust dose-dependent activation response when treated with TNFɑ, or the Protein Kinase C activator Phorbol 12-myristate 13-acetate (PMA). As such, the principle application of this assay system is in the screening of test samples to quantify any functional activities that they may exert to modulate, either induce or suppress, NF-κB activities. INDIGO Bioscience’s assay kits are all-inclusive cell-based assay systems. In addition to NF-κB Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a positive control activator of NF-κB, Luciferase Detection Reagent, and a cell culture-ready assay plate.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 6 months
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You may be eligible to receive a free sample of Human NF-κB Reporter Assay System under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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