ERRγ (human) Reporter Assay Kit | Cayman Chemical
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ERRγ (human) Reporter Assay Kit

Item № 16524
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     96 wells $715.00 0.00
     3 x 32 wells $735.00 0.00

Pricing updated 2018-12-14. Prices are subject to change without notice.

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  • Screen test samples to quantify functional activity, either agonist or antagonist, that they may exert against ERRγ
  • Robust technology provides sustained response for consistent and reproducible data acquistion
  • Included in kit: Ready-to-use, pre-transfected reporter cells | Optimized media | Reference agonist | Luciferase detection reagent | Cell culture ready assay plate
  • Estrogen-Related Receptor γ
  • NR3B3

This nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide high-level expression of a hybrid form of the Human Estrogen-Related Receptor γ (NR3B3). The N-terminal DNA binding domains (DBD) of the native ERRγ has been substituted with that of the yeast GAL4-DBD. The reporter gene is beetle luciferase functionally linked to the GAL4 upstream activation sequence (UAS). As is true in vivo, these reporter cells express ERRγ that is constitutively active in the (putative) absence of a ligand binding event. Interestingly, the ligand binding domain of ERRγ may be occupied by a ligand that further elevates the constitutive activity of the receptor (an agonist response), or one that results in a dose-dependent loss of constitutive active (an inverse-agonist response).1 Figure 2 demonstrates the high constitutive activity of ERRγ in the absence of added ligand, as well as agonist and inverse-agonist responses of the receptor upon ligand binding. Therefore, the principle application of this assay system is in the screening of test samples to quantify either agonist or inverse-agonist activities that they may exert against human ERRγ. ERRγ Reporter Cells are prepared using INDIGO's proprietary CryoMiteTM process. This cryo-preservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spin-and-rinse of cells, viability determinations, cell titer adjustments, or the preincubation of reporter cells prior to assay setup. INDIGO Bioscience's Nuclear Receptor Reporter Assays are all-inclusive cell-based assay systems. In addition to ERRγ Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference agonist, Luciferase Detection Reagent, and a cell culture-ready assay plate. [INDIGO Catalog No. IB08021]

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Estrogen-Related Receptor γ
  • NR3B3

Warning - this product is not for human or veterinary use.

Shipping & Storage
Dry ice in continental US; may vary elsewhere
≥ 6 months
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Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Wang, L., Zuercher, W.J., Consler, T.G., et al. X-ray crystal structures of the estrogen-related receptor- g ligand binding domain in three functional states reveal the molecular basis of small molecule regulation The Journal of Biological Chemisty 281, 37773-37781 (2006).

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