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mOrange mRNA (Cap-1; m1Ψ) encodes mOrange, an orange fluorescent protein and derivative of Discosoma red fluorescent protein (DsRed) that displays excitation and emission maxima to 548 and 562 nm, respectively.1 It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap-1 structure with high capping efficiency. mOrange mRNA is also polyadenylated and modified with N1-methylpseudouridine (m1Ψ) to reduce the host cell immune response and enhance mRNA stability. Encapsulation of mOrange mRNA (Cap-1; m1ψ) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of mOrange.
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1. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22(12), 1567-1572 (2004).