A competitive ELISA for the quantification of PGE2
Features
  • Measure PGE2 in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Detect PGE2 levels down to 11 pg/ml
  • Rapid assay; get results in under 4 hours
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Prostaglandin E2 Express ELISA Kit

Item No. 500141

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dinoprostone
  • PGE2 Express
  • PGE2 Express EIA
Assay Range
15.6-2,000 pg/ml
Sensitivity
80% B/B0: 40 pg/ml
Cross Reactivity
(Prostaglandin E2) 100%(Prostaglandin E2 Ethanolamide) 100%(Prostaglandin E2-1-glyceryl ester) 100%(Prostaglandin E3) 43.0%(Prostaglandin E1) 18.7%(8-iso Prostaglandin E2) 2.5%(Sulprostone) 1.3%(6-keto Prostaglandin F1α) 1.0%(8-iso Prostaglandin F2α) 0.25%(Prostaglandin A2) 0.04%(13,14-dihydro-15-keto Prostaglandin E2) <0.01%(Arachidonic acid) <0.01%(Arachidonoyl Ethanolamide) <0.01%(O-Arachidonoyl Ethanolamide) <0.01%(Butaprost) <0.01%(Conjugated Linoleic Acid (10E,12Z)) <0.01%(8(S),15(S)-DiHETE) <0.01%(Leukotriene B4) <0.01%(Misoprostol) <0.01%(Misoprostol (free acid)) <0.01%(tetranor-PGEM) <0.01%(tetranor-PGFM) <0.01%(Prostaglandin A1) <0.01%(8-iso Prostaglandin A2) <0.01%(Prostaglandin A3) <0.01%(Prostaglandin B1) <0.01%(Prostaglandin B2) <0.01%(Prostaglandin D2) <0.01%(11-deoxy Prostaglandin E2) <0.01%(16,16-dimethyl Prostaglandin E2) <0.01%(19(R)-hydroxy Prostaglandin E2) <0.01%(20-hydroxy Prostaglandin E2) <0.01%(15-keto Prostaglandin E2) <0.01%(Prostaglandin F1α) <0.01%(Prostaglandin F2α) <0.01%(13,14-dihydro-15-keto Prostaglandin F2α) <0.01%(Prostaglandin F3α) <0.01%(Prostaglandin J2) <0.01%(15-deoxy-.DELTA.12,14-Prostaglandin J2) <0.01%(Thromboxane B2) <0.01%
Lower Limit of Quantification
11 pg/ml
Origin
Animal/Bovine, Animal/Eel, Animal/Goat, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Cayman’s PGE2 Express ELISA is a competitive assay that can be used for quantification of PGE2 in urine, plasma, serum, tissue homogenates, and culture media samples. The assay has a range of 15.6-2,000 pg/ml, a sensitivity (80% B/B0) of approximately 40 pg/ml, and a lower limit of detection (LLOD) of 11 pg/ml.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Citations

    Miyamoto, J., Igarashi, M., Watanabe, K., et al. Gut microbiota confers host resistance to obesity by metabolizing dietary polyunsaturated fatty acids. Nat. Commun. 10(1), 4007 (2019).

    Sakumoto, R., Hayashi, K.G., Fujii, S., et al. Possible roles of CC- and CXC-chemokines in regulating bovine endometrial function during early pregnancy. Int. J. Mol. Sci. 18(4), E742 (2017).

    Li, C., Srivastava, R.K., Weng, Z., et al. Molecular mechanism underlying pathogenesis of lewisite-induced cutaneous blistering and inflammation: Chemical chaperones as potential novel antidotes. Am. J. Pathol. 186(10), 2637-2649 (2016).

    Wu, L.-Q., Li, Y., Li, Y.-Y., et al. Antinociceptive effects of prim-O-glucosylcimifugin in inflammatory nociception via reducing spinal COX-2. Biomol. Ther. (Seoul) 24(4), 418-425 (2016).

    Liu, Y.-W., Mei, H.-C., Su, Y.-W., et al. Inhibitory effects of Pleurotus tuber-regium mycelia and bioactive constituents on LPS-treated RAW 264.7 cells. J. Funct. Foods 7, 662-670 (2014).

    Barton, M.H., Paske, E., Norton, N., et al. Efficacy of cyclo-oxygenase inhibition by two commercially available firocoxib products in horses. Equine Vet. J. 46(1), 72-75 (2014).

    Farhat, A., Philibert, P., Sultan, C., et al. Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology. J. Ovarian Res. 4, 3 (2011).

    Kim, D.W., Chi, Y.S., Son, K.H., et al. Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response. Arch. Pharm. Res. 25(3), 329-335 (2002).