Thromboxane B2 ELISA Kit

Item № 501020
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $285.00 0.00
96 strip wells $285.00 0.00
480 solid wells $1,065.00 0.00
480 strip wells $1,065.00 0.00

Pricing updated 2018-02-22. Prices are subject to change without notice.

Description
Features
  • Measure TXB2 in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure TXB2 levels down to 5 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • TXB2 EIA Kit

Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and contraction of vascular and bronchial smooth muscle.1,2,3 Like most lipid mediators, TXA2 is not a circulating hormone. It is formed in response to local stimuli and exerts its effects within a short distance of its biosynthesis. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2, which is then quickly metabolized (t½ = 5-7 minutes) to urinary metabolites for clearance by the kidneys.4 Because of the transient nature of this compound it is difficult to accurately measure circulating levels in whole animal experimental models. In fact, it has been shown that plasma and urine levels of TXB2 are primarily due to ex vivo platelet activation and intra-renal production, respectively.4,5,6 Therefore, measurement of TXB2 metabolites, such as 11-dehydro TXB2 (Item No. 519510), in urine and plasma may give more accurate estimates of in vivo TXA2 production.7,8,9 TXB2 measurement is better suited for samples that are unlikely to undergo extensive metabolism such as perfusates, lavage samples, tissue/cell culture, etc.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • TXB2 EIA Kit
Assay Range
1.6-1,000 pg/ml
Sensitivity
80% B/B0: 5 pg/ml
Mid-point
50% B/B0: 40-80 pg/ml
Cross Reactivity
  • (Thromboxane B2) 100%
  • (Thromboxane B3) 33.8%
  • (2,3-dinor Thromboxane B2) 18.5%
Show all 15

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Hamberg, M.,Svensson, J., and Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proceedings of the National Academy of Sciences of the United States of America 72, 2994-2998 (1975).

2. Ellis, E.F.,Oelz, O.,Roberts, L.J., II, et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A2. Science 193, 1135-1137 (1976).

3. Salzman, P.M.,Salmon, J.A., and Moncada, S. Prostacyclin and thromboxane A2 synthesis by rabbit pulmonary artery. Journal of Pharmacology and Experimental Therapeutics 215, 240-247 (1980).

4. Patrono, C.,Ciabattoni, G.,Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. Journal of Clinical Investigation 77, 590-594 (1986).

5. Patrono, C.,Ciabattoni, G.,Patrignani, P., et al. Evidence for a renal origin of urinary thromboxane B2 in health and disease. Advances in Prostaglandin, Thromboxane, and Leukotriene Research 11, 493-498 (1983).

6. Samuelsson, B.,Granström, E.,Green, K., et al. Prostaglandins. Annual Reviews of Biochemistry 44, 669-695 (1975).

7. Catella, F.,Healy, D.,Lawson, J.A., et al. 11-dehydro Thromboxane B2: A quantitative index of thromboxane A2 formation in the human circulation. Proceedings of the National Academy of Sciences of the United States of America 83, 5861-5865 (1986).

8. Ciabattoni, G.,Pugliese, F.,Davi, G., et al. Fractional conversion of thromboxane B2 to urinary 11-dehydrothromboxane B2 in man. Biochimica et Biophysica Acta 992, 66-70 (1989).

9. Murphy, R.C., and Fitzgerald, G.A. Current approaches to estimation of eicosanoid formation in vivo. Advances in Prostaglandin, Thromboxane, and Leukotriene Research 22, 341-348 (1994).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Thromboxane B2 ELISA Kit

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View the pre-configured analysis tool for Thromboxane B2 ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Thromboxane B2 ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640