Cyclic AMP Select ELISA Kit | Cayman Chemical
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Cyclic AMP Select ELISA Kit

Item № 501040
product image
     96 solid wells $299.00 0.00
     96 strip wells $299.00 0.00
     480 solid wells $1,121.00 0.00
     480 strip wells $1,121.00 0.00

Pricing updated 2018-12-17. Prices are subject to change without notice.

  • A sensitive assay for cAMP in multiple sample matricies without the need for acetylation
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure cAMP levels down to 0.6 pmol/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate
  • cAMP EIA Kit

Cayman’s cAMP Select assay is a competitive ELISA that permits cAMP measurements within the standard curve range of 0.09-200 pmol/ml and a sensitivity (80% B/B0) of approximately 0.6 pmol/ml. Acetylation of samples is not required in this highly sensitive assay where culture media, urine, tissue, and plasma samples have been validated.

Cyclic AMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate
  • cAMP EIA Kit
Assay Range
0.09-200 pmol/ml
80% B/B0: 0.6 pmol/ml
50% B/B0: 4-8 pmol/ml
Cross Reactivity
  • (cAMP) 100%
  • (cGMP) <0.01%
  • (AMP) <0.01%
Show all 6
  • Animal/Bovine
  • Animal/Eel
  • Animal/Mouse
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Cyclic AMP Select ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640