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Vitamin D ELISA Kit

Item № 501050
product image
     96 solid wells $395.00 0.00
     96 strip wells $395.00 0.00
     480 solid wells $1,480.00 0.00
     480 strip wells $1,480.00 0.00

Pricing updated 2019-07-18. Prices are subject to change without notice.

  • Measure vitamin D in plasma or serum
  • Detect the metabolically stable forms 25(OH)D3 and 25(OH)D2
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure vitamin D levels down to 0.5 ng/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Vitamin D EIA Kit

The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)2D3), the biologically active form. Active 1,25(OH)2D3 is very short-lived and is rapidly metabolized to its deactivated forms.

Vitamin D Immunoassay:
Accurate Vitamin D measurement in plasma or serum

Cayman’s Vitamin D EIA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D3 and 25(OH)D2. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Vitamin D EIA Kit
Assay Range
0.19-25 ng/ml
80% B/B0: 0.5 ng/ml
50% B/B0: 1-4 ng/ml
Cross Reactivity
  • (25-OH Vitamin D3) 100%
  • (1,25-(OH)2 Vitamin D2) 265%
  • (25-OH Vitamin D2) 200%
Show all 8
  • Animal/Bovine
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Krejner, A., Litwiniuk, M., and Grzela, T. LL-37 but not 25-hydroxy-vitamin D serum level correlates with healing of venous leg ulcers Arch. Immunol. Ther. Exp. (Warsz.) Epub ahead of print, (2016).

Zhang, L., Chen, X., Qu, J., et al. The effects of different intensities of exercise and active vitamin D on mouse bone mass and bone strength J. Bone Miner. Metab. Epub ahead of print, (2016).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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