A competitive ELISA for the quantification of 8-OHdG and 8-OHG
Features
  • Measure DNA oxidative damage marker 8-hydroxy-2’-deoxyguanosine and RNA damage marker 8-hydroxyguanosine with equal selectivity and sensitivity
  • Cross reactivity of clone 7E6.9 validated by mass spectrometry
  • See our poster for more information:Critical comparison of three 8-hydroxy-2’-deoxyguanosine monoclonal antibodies
  • Assay 24 samples (urine or other matrices) in triplicate or 36 samples in duplicate
  • Measure 8-hydroxyguanosine levels down to 0.45 ng/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
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DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit

Item No. 501130

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DNA/RNA Oxidative Damage (Clone 7E6.9) EIA Kit
  • 8-Hydroxyguanosine
  • 8-OHG
Assay Range
0.15-20 ng/ml
Sensitivity
80% B/B0: 0.45 ng/ml
Cross Reactivity
(8-hydroxy-2'-deoxyguanosine) 100%(8-hydroxyguanosine) 100%(8-hydroxyguanine) 0.15%(Guanine) <0.1%(Guanosine) <0.1%
Origin
Animal/Bovine, Animal/Eel, Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

    $480

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    96 strip wells

    $480

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    Product Description

    DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer.1,2,3 Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman's DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit is a competitive assay that can be used to measure both 8-OHdG and 8-OHG. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging. Free Radic. Biol. Med. 52, 1700-1707 (2012).

    2. Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging. Free Radic. Res. 46(9), 1093-1098 (2012).

    3. Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy. Cancer Epidemiol. Biomarkers Prev. 21, 629-634 (2012).

    Product Citations

    Yoon, S.P., and Kim, J. Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity. Anat. Cell Biol. 49, 165-176 (2016).