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DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit

Item № 501130
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $375.00 0.00
     96 strip wells $375.00 0.00
     480 solid wells $1,500.00 0.00
     480 strip wells $1,500.00 0.00

Pricing updated 2019-03-20. Prices are subject to change without notice.

Description
Features
  • Measure DNA oxidative damage marker 8-hydroxy-2’-deoxyguanosine and RNA damage marker 8-hydroxyguanosine with equal selectivity and sensitivity
  • Cross reactivity of clone 7E6.9 validated by mass spectrometry
  • See our poster for more information: "Critical comparison of three 8-hydroxy-2’-deoxyguanosine monoclonal antibodies."
  • Assay 24 samples (urine or other matrices) in triplicate or 36 samples in duplicate
  • Measure 8-hydroxyguanosine levels down to 0.45 ng/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • DNA/RNA Oxidative Damage (Clone 7E6.9) EIA Kit
  • 8-Hydroxyguanosine
  • 8-OHG

DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer.1,2,3 Guanine is the base is most prone to oxidation, and the repair processes initiated to correct the damage release multiple oxidized guanine species into the urine, including 8-hydroxyguanosine (8-OHG), 8-hydroxy-2’-deoxyguanosine (8-OHdG), and 8-hydroxyguanine. Some commercial vendors offer immunoassays that detect only 8-OHdG. Cayman's DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit is a competitive assay that can be used to measure both 8-OHdG and 8-OHG. Since the antibody used in the assay recognizes damaged nucleic acid species other than 8-OHdG, the value obtained with our assay will be higher than that obtained by competitor ELISAs or by LC/MS analysis that measure a single species. As such, caution is recommended when comparing results obtained from this kit to those obtained by other methods.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • DNA/RNA Oxidative Damage (Clone 7E6.9) EIA Kit
  • 8-Hydroxyguanosine
  • 8-OHG
Assay Range
0.15-20 ng/ml
Sensitivity
80% B/B0: 0.45 ng/ml
Mid-point
50% B/B0: 1.25-1.75 ng/ml
Cross Reactivity
  • (8-hydroxy-2'-deoxyguanosine) 100%
  • (8-hydroxyguanosine) 100%
  • (8-hydroxyguanine) 0.15%
Show all 5
Origin
  • Animal/Bovine
  • Animal/Eel
  • Animal/Mouse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging Free Radical Biology & Medicine 52, 1700-1707 (2012).

2. Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging Free Radical Research 46(9), 1093-1098 (2012).

3. Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy Cancer Epidemiology, Biomarkers & Prevention 21, 629-634 (2012).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

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You may be eligible to receive a free sample of DNA/RNA Oxidative Damage (Clone 7E6.9) ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640