For the quantification of pregnanediol-3-glucuronide in urine
Features
  • Measures pregnanediol-3-glucuronide, the primary urinary metabolite of progesterone
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure pregnanediol-3-glucuronide levels down to 0.23 ng/ml
  • Incubation: 2 hours | Development: 60 minutes | Read: Colorimetric at 405 nm
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Pregnanediol-3-Glucuronide (PDG) ELISA Kit

Item No. 501300

Kit Booklet (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PDG
  • Pregnanediol-3-Glucuronide (PDG) EIA Kit
Limit of Detection
0.23 ng/ml
Assay Range
0.4-50 ng/ml
Sensitivity
0.94 ng/ml
Cross Reactivity
(100%) Pregnanediol-3-Glucuronide(25.8%) Progesterone(0.03%) Corticosterone(0.02%) DHEA Sulfate(0.01%) Estriol(0.002%) Estradiol Glucuronide(0.006%) Aldosterone(0.009%) Cortisol(0.35%) Testosterone(0.006%) Testosterone Glucuronide
Origin
Animal/Bovine, Animal/Mouse, Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    96 solid wells

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    Product Description

    Pregnanediol-3-Glucuronide (5β-pregnane-3α,20α-diol-3α-glucuronide, PDG or P3G) is the major metabolite of progesterone, an endogenous steroid and the most important member of the class of steroid hormones known as progestogens. Progesterone is important in a variety of biological functions such as preparing the endometrium for implantation, maintaining pregnancy, differentiating breast tissue, and promoting normal development of neurons in the brain as a neuro steroid. Low progesterone levels are linked to chronic anovulation which is reported to be associated with female infertility, breast cancer and endometrial cancer.1,2 Levels of PDG in the urine correlate highly to levels of progesterone measured in the serum.3,4 PDG analysis may be used as a non-invasive method to indirectly measure progesterone and therefore might be used as a biomarker for luteal activity, ovarian cancer, and a variety of other biological disorders.5,6,7

    Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kassam, A., Overstreet, J.W., Snow-Harter, C., et al. Identification of anovulation and transient luteal function using a urinary pregnanediol-3-glucuronide ratio algorithm. Environ. Health Perspect. 104(4), 408-413 (1996).

    2. Wiebe, J.P. Progesterone metabolites in breast cancer. Endocr. Relat. Cancer 13(3), 717-738 (2006).

    3. O'Connor, K.A., Brindle, E., Holman, D.J., et al. Urinary estrone conjugate and pregnanediol 3-glucuronide enzyme immunoassays for population research. Clin. Chem. 49(7), 1139-1148 (2003).

    4. Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin. Chem. 37(6), 838-844 (1991).

    5. Pavlovic, J.M., Allshouse, A.A., Santoro, N.F., et al. Sex hormones in women with and without migraine: Evidence of migraine-specific hormone profiles. Neurology 87(1), 49-56 (2016).

    6. Lukanova, A., and Kaaks, R. Endogenous hormones and ovarian cancer: Epidemiology and current hypotheses. Can. Epidemiol Biomarkers Prev. 14(1), 98-107 (2005).

    7. Kim, J.J., Kurita, T., and Bulun, S.E. Progesterone action in endometrial cancer, endometriosis, uterine fibroids, and breast cancer. Endocr. Rev. 34(1), 130-162 (2013).