2'3'-cGAMP ELISA Kit | Cayman Chemical
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2'3'-cGAMP ELISA Kit

Item № 501700
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $425.00 0.00
     96 strip wells $425.00 0.00

Pricing updated 2018-07-16. Prices are subject to change without notice.

Description
Features
  • Measure 2'3'-cGAMP in cell lysates
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Lower limit of detection (LLOD) is 9.6 pg/ml (0.01 pmol/ml)
  • Run with overnight or 2-hour incubation protocols without compromising sensitivity
  • Monitor the kinetics of 2'3'-cGAMP formation and hydrolysis in a biological setting
  • Identify compounds that modulate cGAS activity
Synonyms
  • 2'3'-cGAMP EIA Kit
  • 2'3'-Cyclic GMP-AMP
  • Guanosine-Adenosine 2',3'-cyclic monophosphate

Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that detects the presence of nucleic acids in the cytosol of mammalian cells as an indicator of bacterial or viral infection.1 cGAS catalyzes the synthesis of a second messenger, 2’3’-cGAMP, from cytosolic ATP and GTP in response to dsDNA binding. 2’3’-cGAMP then binds tightly to the adaptor protein STING (stimulator of interferon genes), resulting in the recruitment of TBK1 and subsequent IRF3 phosphorylation.2 IRF3 induces the transcription and translation type I interferon, a potent antiviral cytokine.3 Activation of cGAS and the production of 2’3’-cGAMP are important in host defense, but also may play role in autoimmune or inflammatory diseases. Modulation of cGAS activity, with subsequent inhibition of induction of 2’3’-cGAMP formation is an active target of pharmacological intervention.4

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • 2'3'-cGAMP EIA Kit
  • 2'3'-Cyclic GMP-AMP
  • Guanosine-Adenosine 2',3'-cyclic monophosphate

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

2. Hall, J., Brault, A., Vincent, F., et al. Discovery of PF-06928215 as a high affinity inhibitor of cGAS enabled by novel fluorescence polarization assay. PLoS One 12(9), e0184843 (2017).

3. O'Neill, L.A. Sensing the dark side of DNA. Science 339(6121), 763-764 (2013).

4. Cai, X., Chiu, Y.-H., and Chen, Z.J. The cGAS-cGAMP-STING pathway of cytosolic DNA sensing and signaling. Mol. Cell 54(2), 289-296 (2014).

Technical Support
Analysis Tools

View our analysis workbook for 2'3'-cGAMP ELISA Kit

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