Prostaglandin D2-MOX ELISA Kit | Cayman Chemical

Prostaglandin D2-MOX ELISA Kit

Item № 512011
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $270.00 0.00
     96 strip wells $270.00 0.00
     480 solid wells $1,013.00 0.00
     480 strip wells $1,013.00 0.00

Pricing updated 2018-05-24. Prices are subject to change without notice.

Description
Features
  • Treatment of samples with MOX hydrochloride converts unstable PGD2 to stable PGD2-MOX
  • Highly sensitive
  • Measure PGD2-MOX levels down to 3.1 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • PGD2-MOX EIA Kit

Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kD glutathione-independent lipocalin-type PGD2 synthase.1 PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans.2 PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase. This PGD2 which is formed in the intracellular and vascular compartments is rapidly metabolized to 11β-PGF.3 Thus, urinary measurements of PGD synthesis are most appropriately focused on the measurement of 11β-PGF. Measurement of the parent eicosanoid PGD2 is appropriate in the supernatants of cell cultures, where PGD2 levels may reach several ng/ml, and in CSF, where concentrations of several hundred pg/ml have been measured.4 All studies of PGD2 biosynthesis should take into consideration the chemical instability of PGD2 and its rapid degradation in the presence of serum proteins such as albumin.5 PGD2 also readily degrades in both acidic and basic media to give a variety of decomposition products. Similarly, antigenic protein conjugates of PGD2, synthesized for the production of antisera, show considerable amounts of decomposition. Thus, the resulting antibody response is heterogeneous with poor specificity. This makes PGD2 assay systems based on the parent compound unreliable and difficult to interpret. This PGD2-MOX ELISA is based on the conversion of PGD2 to a stable MOX derivative. Treatment of the sample with methoxylamine hydrochloride (MOX HCl) converts PGD2 into PGD2-MOX, preventing its further chemical degradation. The antiserum used in the assay was developed using conjugates of this derivative and is very specific for PGD2-MOX. The assay has been validated against stable isotope dilution GC/MS. Measurements using both techniques on identical samples showed a correlation coefficient of 0.97.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Recommended Products

Related Products
View Related Product Categories
Technical Information
Synonyms
  • PGD2-MOX EIA Kit
Assay Range
2.0-250 pg/ml
Sensitivity
80% B/B0: 3 pg/ml
Mid-point
50% B/B0: 10-35 pg/ml
Cross Reactivity
  • (Prostaglandin F) <0.01%
  • (tetranor-PGEM) <0.01%
  • (tetranor-PGFM) <0.01%
Show all 8

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. The Journal of Biological Chemisty 270, 1422-1428 (1995).

2. Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neuroscience Research 21, 41-50 (1995).

3. Roberts, L.J., II, and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30(3), 383-400 (1985).

4. Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D2 formation and characterization of its synthetases in various tissues of adult rats. Archives of Biochemistry and Biophysics 260, 521-531 (1988).

5. Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. The Journal of Biological Chemisty 258, 11713-11718 (1983).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Prostaglandin D2-MOX ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for Prostaglandin D2-MOX ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We work with scientists to support their research, drug discovery, and drug development needs through our expertise in the following research services:

Promotions

View all of our current Promotions

You may be eligible to receive a free sample of Prostaglandin D2-MOX ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640