Prostaglandin E2 ELISA Kit - Monoclonal | Cayman Chemical
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Prostaglandin E2 ELISA Kit - Monoclonal

Item № 514010
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $275.00 0.00
     96 strip wells $275.00 0.00
     480 solid wells $1,031.00 0.00
     480 strip wells $1,031.00 0.00

Pricing updated 2018-08-15. Prices are subject to change without notice.

Description
Features
  • Measure PGE2 in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure PGE2 levels down to 15 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • PGE2 EIA Kit - Monoclonal

Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo, PGE2 is rapidly converted to an inactive metabolite (13,14-dihydro-15-keto PGE2) by the prostaglandin 15-dehydrogenase pathway.1,2 The half-life of PGE2 in the circulatory system is approximately 30 seconds and normal plasma levels are 3-12 pg/ml.3 Our PGE2 ELISA has been validated for use with urine, plasma, and culture media samples. In general, urine and culture media samples can be diluted, if necessary, and added directly to the assay well. Plasma samples should be purified prior to use. Because of the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is often best accomplished by the measurement of PGE2 metabolites. Our Prostaglandin E Metabolite assay (Item No. 514531) converts all major PGE2 metabolites into a single stable derivative which is easily measurable by ELISA.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • PGE2 EIA Kit - Monoclonal
Assay Range
7.8-1,000 pg/ml
Sensitivity
80% B/B0: 15 pg/ml
Mid-point
50% B/B0: 30-70 pg/ml
Cross Reactivity
  • (Prostaglandin E2) 100%
  • (Prostaglandin E2 Ethanolamide) 100%
  • (Prostaglandin E2-1-glyceryl ester) 100%
Show all 40

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19(6), 933-945 (1980).

2. Hamberg, M., and Samuelsson, B. On the metabolism of prostaglandins E1 and E2 in man. J. Biol. Chem. 246(22), 6713-6721 (1971).

3. Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE2. Prostaglandins 19(6), 917-931 (1980).

Product Citations

Arcanjo, A.F., Nunes, M.P., Silva-Junior, E.B., et al. B-1 cells modulate the murine macrophage response to Leishmania major infection.. World J. Biol. Chem. 8(2), 151-162 (2017).

Lyons, T.R., Borges, V.F., Betts, C.B., et al. Cyclooxygenase-2-dependent lymphangiogenesis promotes nodal metastasis of postpartum breast cancer. J. Clin. Invest. 124(9), 3901-3912 (2014).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Prostaglandin E2 ELISA Kit - Monoclonal

View all analysis workbooks

View the pre-configured analysis tool for Prostaglandin E2 ELISA Kit - Monoclonal on MyAssays.com

FAQ

What is the effect of BSA in samples in the Prostaglandin E2 EIA Kit - Monoclonal?

The EIA buffer, the medium that the Prostaglandin E2 EIA Kit - Monoclonal is performed in, contains 0.1% BSA as its working concentration. Also samples are diluted in this buffer. Unless you are planning on using significantly higher levels of BSA, your samples should be okay. Assaying all samples at two dilutions is recommended.

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You may be eligible to receive a free sample of Prostaglandin E2 ELISA Kit - Monoclonal under the Cayman Challenge program.

Cayman Chemical

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Ann Arbor, Michigan 48108 USA

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