Prostaglandin E Metabolite ELISA Kit

Item № 514531
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $275.00 0.00
96 strip wells $275.00 0.00
480 solid wells $1,031.00 0.00
480 strip wells $1,031.00 0.00

Pricing updated 2018-02-18. Prices are subject to change without notice.

Description
Features
  • Converts major urinary and plasma metabolites of PGE2 to a single, stable derivative that is easily quantified
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure PGEM levels down to 2 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • PGE Metabolite EIA Kit
  • PGEM

Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2 from the medium is a good way to measure PGE2 production from these cells. PGE2 is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE2 cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE2, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE2 production. Cayman’s PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA2 and 13,14-dihydro-15-keto PGE2 to a single, stable derivative that could be easily quantified by ELISA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • PGE Metabolite EIA Kit
  • PGEM
Assay Range
0.39-50 pg/ml
Sensitivity
80% B/B0: ~2 pg/ml
Mid-point
50% B/B0: ~8-14 pg/ml
Cross Reactivity
  • (13,14-dihydro-15-keto Prostaglandin E1 (derivatized)) 100%
  • (13,14-dihydro-15-keto Prostaglandin E2 (derivatized)) 100%
  • (Bicyclo Prostaglandin E1) 38%
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Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Wang, H.,Sethi, G.,Loke, W.-K., et al. Des-aspartate-angiotensin I attenuates mortality of mice exposed to gamma radiation via a novel mechanism of action. PLoS One 10(9), e0138009 (2015).

Richardson, G.F.,McNiven, M.A.,Petit, H.V., et al. The effects of dietary omega fatty acids on pregnancy rate, plasma prostaglandin metabolite levels, serum progesterone levels, and milk fatty-acid profile in beef cows. Can. J. Vet. Res. 77(4), 314-318 (2013).

Petit, H.V.,Small, J.A.,Palin, M.F., et al. Effects of flaxseed supplementation on endometrial expression of ISG17 and intrauterine prostaglandin concentrations in primiparous dairy cows submitted to GnRH-based synchronized ovulation. Can. J. Animal Sci. 87(3), 343-352 (2007).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Prostaglandin E Metabolite ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for Prostaglandin E Metabolite ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Prostaglandin E Metabolite ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640