Prostaglandin F ELISA Kit

Item № 516011
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $285.00 0.00
96 strip wells $285.00 0.00
480 solid wells $1,069.00 0.00
480 strip wells $1,069.00 0.00

Pricing updated 2018-02-25. Prices are subject to change without notice.

Description
Features
  • Measure PGF in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure PGF levels down to 10 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • PGF EIA Kit

Prostaglandin F (PGF) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF is rapidly metabolized to 13,14-dihydro-15-keto PGF in vivo, and the levels of this metabolite in both plasma and amniotic fluid are elevated during active labor in several different mammalian species. Like all of the primary PGs, PGF. has a very short half-life in the general circulation. The plasma concentration of PGF in humans is <10 pg/ml, and probably no more than 1-2 pg/ml.1 PGF is rapidly metabolized by 15-hydroxy PG dehydrogenase and both β- and ω-oxidation systems to a variety of polar metabolites.2 In primary cultures, the metabolism of PGF is much more limited, and it may collect in the supernatant medium to easily measured concentrations.3 Amnionic and placental tissue PGF content in the range from 100-250 ng/g of tissue has been reported.4 However, this study did not address the presence of isoprostanes and reported measurements using an unvalidated RIA method. A controlled GC- or LC-MS study of tissue PG content has not been reported. Tissue PG measurements in general are difficult to perform, vary widely, and are challenging to interpret.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • PGF EIA Kit
Assay Range
3.9-500 pg/ml
Sensitivity
80% B/B0: 10 pg/ml
Mid-point
50% B/B0: 40-60 pg/ml
Cross Reactivity
  • (Prostaglandin F Ethanolamide) 244.1%
  • (Prostaglandin F) 100%
  • (Prostaglandin F) 61%
Show all 13

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Schweer, H.,Kammer, J. and Seyberth, H.W. Simultaneous determination of prostanoids in plasma by gas chromatography-negative-ion chemical ionization mass spectrometry. Journal of Chromatography 338, 273-280 (1985).

2. Granström, E. and Kindahl, H. Species differences in circulating prostaglandin metabolites relevance for the assay of prostaglandin release. Biochimica et Biophysica Acta 713, 555-569 (1982).

3. Ritzi, E.M.,Boto, W.O. and Stylos, W.A. Measurement of initial prostaglandin F metabolites in medium of BALB/c 3T3 and SV3T3 mouse fibroblast cultures. Biochemical and Biophysical Research Communications 63, 179-186 (1975).

4. Reece, M.S.,McGregor, J.A.,Allen, K.G.D., et al. Prostaglandins in selected reproductive tissues in preterm and full term gestations. Prostaglandins, Leukotrienes and Essential Fatty Acids 55, 303-307 (1996).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Prostaglandin F ELISA Kit

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View the pre-configured analysis tool for Prostaglandin F ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Prostaglandin F ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640