11β-Prostaglandin F2α ELISA Kit | Cayman Chemical
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11β-Prostaglandin F ELISA Kit

Item № 516521
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $246.00 0.00
     96 strip wells $246.00 0.00
     480 solid wells $923.00 0.00
     480 strip wells $923.00 0.00

Pricing updated 2018-06-22. Prices are subject to change without notice.

Description
Features
  • Detect 11β-PGF, the primary metabolite of PGD2, in plasma or urine
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 11β-PGF levels down to 5 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • 11β-PGF EIA Kit

Prostaglandin D2 (PGD2) is the major eicosanoid released from stimulated mast cells. PGD2 is an unstable compound which is rapidly metabolized and eliminated from the circulation. This makes quantitation of PGD2 an unreliable indicator of its in vivo production. 11β-PGF is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis.1 The normal human urinary excretion of 11β-PGF is about 11 ng/mmol creatinine (~400 ng/24 hr), which is increased nearly 3-fold upon allergen-induced bronchoconstriction in asthmatics.2,3 Unlike most prostaglandin metabolites, 11β-PGF retains potent biological activity. 11-β-PGF is equipotent to PGF in inducing human bronchial smooth muscle contractions and inhibition of adipose differentiation.4,5 11β-PGF was also shown to inhibit ADP or thrombin-induced human platelet aggregation at concentrations of 0.14 to 2.8 µM. 6 The Cayman 11β-PGF Assay is a competitive assay that provides accurate measurements of 11β-PGF within the range of 1.6-1,000 pg/ml. Inter and intra-assay CV’s of less than 15% can be achieved at most concentrations. This assay allows sensitive detection of 11β-PGF in the most common sample matrix, which is urine. For plasma and other complex sample matrices, we recommend purification of the sample prior to analysis; a purification protocol is included in the kit booklet. Measurements of 11β-PGF in urine using our ELISA gives values in the same range, but slightly higher than those obtained by GC-MS. This appears to be due to measurement of 11β-PGF plus the 2,3-dinor metabolite of 11β-PGF.2

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • 11β-PGF EIA Kit
Assay Range
1.6-1,000 pg/ml
Sensitivity
80% B/B0: 5 pg/ml
Mid-point
50% B/B0: 25-45 pg/ml
Cross Reactivity
  • (11β-Prostaglandin F) 100%
  • (2,3-dinor-11β-Prostaglandin F) 10%
  • (11β-13,14-dihydro-15-keto Prostaglandin F) 0.5%
Show all 15

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Liston, T.E., and Roberts, L.J., II. Transformation of prostaglandin D2 to 9α,11β-(15S)-trihydroxy-prosta-(5Z,13E)-dien-1-oic acid (9α,11β-prostaglandin F2): A unique biologically active prostaglandin produced enzymatically in vivo in humans. Proc. Natl. Acad. Sci. U.S.A. 82(18), 6030-6034 (1985).

2. O'Sullivan, S., Mueller, M.J., Dahlén, S.E., et al. Analyses of prostaglandin D2 metabolites in urine: Comparison between enzyme immunoassay and negative ion chemical ionization gas chromatography-mass spectrometry. Prostaglandins & Other Lipid Mediators 57, 149-165 (1999).

3. O'Sullivan, S., Dahlén, B., Dahlén, S.E., et al. Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced airway obstruction. J. Allergy Clin. Immunol. 98(2), 421-432 (1996).

4. Coleman, R.A., and Sheldrick, R.L.G. Prostanoid-induced contraction of human bronchial smooth muscle is mediated by TP-receptors. Br. J. Pharmacol. 96(3), 688-692 (1989).

5. Lepak, N.M., and Serrero, G. Inhibition of adipose differentiation by 9α, 11β-prostaglandins F. Prostaglandins 46(6), 511-517 (1993).

6. Pugliese, G., Spokas, E.G., Marcinkiewicz, E., et al. Hepatic transformation of prostaglandin D2 to a new prostanoid, 9α,11β-prostaglandin F2, that inhibits platelet aggregation and constricts blood vessels. J. Biol. Chem. 260(27), 14621-14625 (1985).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 11β-Prostaglandin F ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for 11β-Prostaglandin F ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of 11β-Prostaglandin F ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640