13,14-dihydro-15-keto Prostaglandin F ELISA Kit

Item № 516671
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $270.00 0.00
96 strip wells $270.00 0.00
480 solid wells $1,013.00 0.00
480 strip wells $1,013.00 0.00

Pricing updated 2018-02-22. Prices are subject to change without notice.

Description
Features
  • Detect 13,14-dihydro-15-keto PGF, a stable metabolite of PGF
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 13,14-dihydro-15-keto PGF levels down to 16.3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • 13,14-dihydro-15-keto PGF EIA Kit
  • PGFM EIA Kit

Prostaglandin F (PGF) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF is rapidly metabolized to 13,14-dihydro-15-keto PGF in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and Δ13-reductucase.1,2,3,4 Measurement of 13,14-dihydro-15-keto PGF in plasma can be used as a marker of the in vivo production of PGF.5,6 The Cayman Chemical 13,14-dihydro-15-keto PGF Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF within the range of 2.3-5,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 15 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF in plasma, a common matrix for measurement of this PGF metabolite.5

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • 13,14-dihydro-15-keto PGF EIA Kit
  • PGFM EIA Kit
Assay Range
2.3-5,000 pg/ml
Sensitivity
80% B/B0: 15 pg/ml
Mid-point
50% B/B0: 120-180 pg/ml
Cross Reactivity
  • (13,14-dihydro-15-keto Prostaglandin F) 100%
  • (13,14-dihydro-15-keto Prostaglandin E2) 2.7%
  • (15-keto Prostaglandin F) 1.8%
Show all 15

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 6 months
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Samuelsson, B.,Granström, E.,Green, K., et al. Prostaglandins. Annual Reviews of Biochemistry 44, 669-695 (1975).

2. Keirse, M.J.N.C., and Turnbull, A.C. Metabolism of prostaglandins within the pregnant uterus. British Journal of Obstetrics and Gynaecology 82, 887-893 (1975).

3. Basu, S., and Stjernschantz, J. Δ13-reductase dependent metabolism of prostaglandins in the mammalian brain and eye. Prostaglandins, Leukotrienes and Essential Fatty Acids 57, 305-310 (1997).

4. Ensor, C.M.,Yang, J.Y.,Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. The Journal of Biological Chemisty 265, 14888-14891 (1990).

5. Del Vecchio, R.P.,Maxey, K.M., and Lewis, G.S. A quantitative solid-phase enzymeimmunoassay for 13,14-dihydro-15-keto-prostaglandin F in plasma. Prostaglandins 43, 321-330 (1992).

6. Meyer, H.H.D.,Eisele, K., and Osaso, J. A biotin-streptavidin amplified enzyme immunoassay for 13,14-dihydro-15-keto-PGF. Prostaglandins 38, 375-383 (1989).

Product Citations

Richardson, G.F.,McNiven, M.A.,Petit, H.V., et al. The effects of dietary omega fatty acids on pregnancy rate, plasma prostaglandin metabolite levels, serum progesterone levels, and milk fatty-acid profile in beef cows. Can. J. Vet. Res. 77(4), 314-318 (2013).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 13,14-dihydro-15-keto Prostaglandin F ELISA Kit

View all analysis workbooks

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of 13,14-dihydro-15-keto Prostaglandin F ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640