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11-dehydro Thromboxane B2 ELISA Kit - Monoclonal

Item № 519510
product image
     96 solid wells $325.00 0.00
     96 strip wells $325.00 0.00
     480 solid wells $1,219.00 0.00
     480 strip wells $1,219.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Detect 11-dehydro TXB2, a stable urinary metabolite of TXA2 and TXB2
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 11-dehydro TXB2 levels down to 34 pg/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • 11-dehydro TXB2 EIA Kit - Monoclonal
  • 11-keto TXB2

Thromboxane A2 (TXA2), produced from arachidonic acid, causes irreversible platelet aggregation, vascular, and bronchial smooth muscle contraction. TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. However, most of the TXB2 measured in the plasma or urine of healthy individuals is due to ex vivo platelet activation or intra-renal production, and normal concentrations of circulating TXB2 are extremely low (1-2 pg/ml) and highly transient (t½ = 5-7 minutes). 11-dehydro TXB2 is a metabolite of TXB2 (not formed by platelets or the kidney) with a circulating half-life (t½) of 45 minutes. Its measurement in plasma or urine will give a time-integrated indication of TXA2 production and is recommended to estimate TXA2 levels to circumvent measurement complications associated with TXB2. Cayman’s 11-dehydro TXB2 ELISA Kit is a competitive assay that can be used for quantification of 11-dehydro TXB2 in urine and other sample matrices. Normal plasma levels of 11-dehydro TXB2 (1-2 pg/ml) are below the detection limit of this assay (16 pg/ml), so plasma samples must be purified and concentrated prior to analysis. Urine samples should be diluted appropriately before assaying. There is evidence suggesting that 11-dehydro TXB2 can be formed non-enzymatically following platelet activation, so it is important to process plasma samples quickly to prevent artifactual formation of 11-dehydro TXB2. The assay has a range from 15.6-2,000 pg/ml and a sensitivity (80% B/B0) of approximately 34 pg/ml.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • 11-dehydro TXB2 EIA Kit - Monoclonal
  • 11-keto TXB2
Assay Range
15.6-2,000 pg/ml
80% B/B0: 34 pg/ml
50% B/B0: 80-160 pg/ml
Cross Reactivity
  • (11-dehydro Thromboxane B2) 100%
  • (11-dehydro-2,3-dinor Thromboxane B2) 330%
  • (Prostaglandin D2) 0.12%
Show all 7
  • Animal/Bovine
  • Animal/Eel
  • Animal/Goat
  • Animal/Mouse

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Citations

Li, H., Liu, K., Boardman, L.A., et al. Circulating prostaglandin biosynthesis in colorectal cancer and potential clinical significance EBioMedicine 2(2), 165-171 (2015).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 11-dehydro Thromboxane B2 ELISA Kit - Monoclonal

View all analysis workbooks

View the pre-configured analysis tool for 11-dehydro Thromboxane B2 ELISA Kit - Monoclonal on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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Cayman Chemical

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Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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Fax: (734) 971-3640