COX (ovine/human) Inhibitor Screening Assay Kit | Cayman Chemical
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COX (ovine/human) Inhibitor Screening Assay Kit

Item № 560131
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     96 wells $375.00 0.00

Pricing updated 2018-05-28. Prices are subject to change without notice.

Description
Features
  • Screen for inhibitors of ovine COX-1 or human COX-2
  • Directly measure PGF produced in the COX reaction
  • Assay 15 samples for COX-1 or COX-2 in duplicate
  • Plate-based colorimetric measurement (405-420 nm)

Cyclooxygenase (COX, also called Prostaglandin H Synthase or PGHS) enzymes contain both cyclooxygenase and peroxidase activities. COX catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins; the conversion of arachidonic acid to PGH2. It is now well established that there are two distinct isoforms of COX. Cyclooxygenase-1 (COX-1) is constitutively expressed in variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, cyclooxygenase-2 (COX-2). COX-2 is responsible for the biosynthesis of PGs under acute inflammatory conditions.1 This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs. The COX (ovine/human) Inhibitor Screening Assay directly measures PGF produced by SnCl2 reduction of COX-derived PGH2. The prostanoid product is quantified via enzyme immunosorbent assay (ELISA) using a broadly specific antibody that binds to all the major prostaglandin compounds. Thus, the Cayman COX assay is more accurate and reliable than an assay based on peroxidase inhibition. The Cayman COX Inhibitor Screening Assay includes both ovine COX-1 and human recombinant COX-2 enzymes in order to screen isozyme-specific inhibitors. This assay is an excellent tool which can be used for general inhibitor screening, or to eliminate false positive leads generated by less specific methods.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Specificity
  • (100%) Prostaglandin E1
  • (100%) Prostaglandin E2
  • (100%) Prostaglandin F
Show all 27

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Xie, W., Chipman, J.G., Robertson, D.L., et al. Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proceedings of the National Academy of Sciences of the United States of America 88, 2692-2696 (1991).

Technical Support
Analysis Tools

View our analysis workbook for COX (ovine/human) Inhibitor Screening Assay Kit

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View the pre-configured analysis tool for COX (ovine/human) Inhibitor Screening Assay Kit on MyAssays.com

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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of COX (ovine/human) Inhibitor Screening Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640