Cyclic GMP ELISA Kit | Cayman Chemical
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Cyclic GMP ELISA Kit

Item № 581021
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $270.00 0.00
     96 strip wells $270.00 0.00
     480 solid wells $1,069.00 0.00
     480 strip wells $1,069.00 0.00

Pricing updated 2018-11-16. Prices are subject to change without notice.

Description
Features
  • Detect non-acetylated or acetylated cGMP in cell lysates, tissue homogenates, plasma, or urine
  • Measure non-acetylated cGMP levels down to 1 pmol/ml or acetylated cGMP levels down to 0.1 pmol/ml
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Contains acetic anhydride
Synonyms
  • cGMP EIA Kit
  • Guanosine 3',5'-cyclic mononucleotide
  • Guanosine 3',5'-cyclic monophosphate
  • Monosodium-GMP

The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2' hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions.1,2,3 Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106 cells).4,5

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • cGMP EIA Kit
  • Guanosine 3',5'-cyclic mononucleotide
  • Guanosine 3',5'-cyclic monophosphate
  • Monosodium-GMP
Assay Range
0.23-30 pmol/ml
Sensitivity
80% B/B0: 1 pmol/ml
Mid-point
50% B/B0: 3-6 pmol/ml
Cross Reactivity
  • (Acetylated:)
  • (Non-Acetylated:)
  • (Acetylated cGMP) 100%
Show all 15

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Chen, L., Salafranca, M.N., and Mehta, J.L. Cyclooxygenase inhibition decreases nitric oxide synthase activity in human platelets American Journal of Physiology 42, H1854-H1859 (1997).

2. Miller, O.V., and Gorman, R.R. Modulation of platelet cyclic nucleotide content by PGE1 and the prostaglandin endoperoxide PGG2 Journal of Cyclic Nucleotide Research 2, 79-87 (1976).

3. Wu, C.C., Ko, F.N., Kuo, S.C., et al. YC-1 inhibited human platelet aggregation through NO-independent activation of soluble guanylate cyclase British Journal of Pharmacology 116, 1973-1978 (1995).

4. Arima, T., Ohshima, Y., Mizuno, T., et al. Cyclic GMP elevation by 5-hydroxytryptamine is due to nitric oxide derived from endogenous nitrosothiol in NG108-15 cells Biochemical and Biophysical Research Communications 227, 473-478 (1996).

5. Kolb, J.P., Paul-Eugene, N., Damais, C., et al. Interleukin-4 stimulates cGMP production by IFN-γ-activated human monocytes. Involvement of the nitric oxide synthase pathway The Journal of Biological Chemisty 269, 9811-9816 (1994).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Cyclic GMP ELISA Kit

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View the pre-configured analysis tool for Cyclic GMP ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of Cyclic GMP ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640