Estradiol ELISA Kit | Cayman Chemical
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Estradiol ELISA Kit

Item № 582251
product image
     96 solid wells $195.00 0.00
     96 strip wells $195.00 0.00
     480 solid wells $731.00 0.00
     480 strip wells $731.00 0.00

Pricing updated 2018-11-14. Prices are subject to change without notice.

  • Measure estradiol (17β-estradiol) in cell culture medium, plasma, and serum
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure estradiol levels down to 15 pg/ml
  • Incubation: 1 hour | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Estradiol EIA Kit
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol

Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5 Estradiol levels in most rodent samples are extremely low, rising to detectable levels only during the pro-estrus period and in pregnant females.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Estradiol EIA Kit
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
Assay Range
6.6-4,000 pg/ml
80% B/B0: 15 pg/ml
50% B/B0: 40-100 pg/ml
Cross Reactivity
  • (Estradiol) 100%
  • (Estradiol-3-sulfate) 14.5%
  • (Estradiol-3-glucuronide) 14%
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Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology Endocrinology and Metabolism 3rd ed., 973-1015 (1995).

2. Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors Journal of Pharmacology and Experimental Therapeutics 262, 632-637 (1992).

3. Miller, W.L., and Tyrrell, J.B. The adrenal cortex Endocrinology and Metabolism 3rd edition, 555-711 (1995).

4. Vance, D.E. Cholesterol and related derivatives Biochemistry 2nd ed., 725-748 (1988).

5. Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay Clin. Chem. 37(6), 838-844 (1991).

Product Citations

Liu, C., Yu, L., Deng, J., et al. Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish. Aquat. Toxicol. 93(2-3), 131-137 (2009).

Liu, C., Deng, J., Yu, L., et al. Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol Aquat. Toxicol. 96(1), 70-76 (2010).

Liu, X., Ji, K., and Choi, K. Endocrine disruption potentials of organophosphate flame retardants and related mechanisms in H295R and MVLN cell lines and in zebrafish Aquat. Toxicol. 114-115, 173-181 (2012).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Estradiol ELISA Kit

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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

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