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Testosterone ELISA Kit

Item № 582701
product image
     96 solid wells $230.00 0.00
     96 strip wells $230.00 0.00
     480 solid wells $863.00 0.00
     480 strip wells $863.00 0.00

Pricing updated 2019-07-21. Prices are subject to change without notice.

  • Measure testosterone in multiple sample types
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure testosterone levels down to 6 pg/ml
  • Incubation: 2 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Testosterone EIA Kit

Testosterone is the prototypic and predominant circulating androgenic steroid. It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis and at puberty. Testosterone is synthesized from 17α-hydroxy progesterone by the enzymes 17,20-lyase and 17β-hydroxysteroid dehydrogenase in the gonads of both males and females. In many target cells it is reduced to 5α-dihydro testosterone, which mediates many of the biological actions of testosterone. It is then further metabolized to 17β-estradiol by aromatase.1 Serum levels of testosterone range from 0.5 ng/ml in women to approximately 6-10 ng/ml in men, declining with age.2

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Testosterone EIA Kit
Assay Range
3.9-500 pg/ml
80% B/B0: 6 pg/ml
50% B/B0: 20-50 pg/ml
Cross Reactivity
  • (4.7%) Methyl Testosterone
  • (140%) 19-Nortestosterone
  • (100%) Testosterone
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  • Animal/Bovine
  • Animal/Eel
  • Animal/Mouse
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Vance, D.E. Cholesterol and related derivatives Biochemistry 2nd ed., 725-748 (1988).

2. Miller, W.L., and Tyrrell, J.B. The adrenal cortex Endocrinology and Metabolism 3rd edition, 555-711 (1995).

Product Citations

Liu, C., Yu, L., Deng, J., et al. Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic-pituitary-gonadal (HPG) axis of zebrafish. Aquat. Toxicol. 93(2-3), 131-137 (2009).

Liu, C., Deng, J., Yu, L., et al. Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol Aquat. Toxicol. 96(1), 70-76 (2010).

Liu, X., Ji, K., and Choi, K. Endocrine disruption potentials of organophosphate flame retardants and related mechanisms in H295R and MVLN cell lines and in zebrafish Aquat. Toxicol. 114-115, 173-181 (2012).

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Testosterone ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for Testosterone ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Cayman Contract Services

We work with scientists to accelerate their research, drug discovery, and drug development needs through our expertise in the following areas:

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640