11-keto Testosterone ELISA Kit | Cayman Chemical

11-keto Testosterone ELISA Kit

Item № 582751
product image
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     96 solid wells $270.00 0.00
     96 strip wells $270.00 0.00
     480 solid wells $1,013.00 0.00
     480 strip wells $1,013.00 0.00

Pricing updated 2018-05-21. Prices are subject to change without notice.

Description
Features
  • Detect the primary androgenic steroid in fish
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure 11-keto testosterone levels down to 1.3 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • 11-KT EIA Kit

While testosterone is the primary androgenic male steroid found in mammals, 11-keto testosterone (11-KT) is a second key androgenic steroid found in fish. It occurs in males together with testosterone in amounts which vary from less than 1 ng/ml to as much as 50-100 ng/ml, depending on the species and the stage of the reproductive cycle. In the sea bass, testosterone concentrations are generally higher than 11-KT, with peak levels found after the spawning season. 11-KT, on the other hand, remains at levels less than 1 ng/ml but rises abruptly to 4-6 ng/ml during spermiation at the height of the spawning season.1 11-KT also shows individual variations in Arctic char, with dominant males having significantly higher 11-KT levels.2

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • 11-KT EIA Kit
Assay Range
0.78-100 pg/ml
Sensitivity
80% B/B0: 1.3 pg/ml
Mid-point
50% B/B0: 2-10 pg/ml
Cross Reactivity
  • (11-keto Testosterone) 100%
  • (Adrenosterone) 2.9%
  • (4-Androsten-11β,17β-diol-3-one) 0.01%
Show all 6

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Prat, F., Zanuy, S., Carrillo, M., et al. Seasonal changes in plasma levels of gonadal steroids of sea bass, Dicentrarchus labrax L. General and Comparative Endocrinology 78, 361-373 (1990).

2. Elofsson, U.O.E., Mayer, I., Damsgård, B., et al. Intermale competition in sexually mature arctic charr: effects on brain monoamines, endocrine stress responses, sex hormone levels, and behavior. General and Comparative Endocrinology 118, 450-460 (2000).

Product Citations

Liu, X., Ji, K., and Choi, K. Endocrine disruption potentials of organophosphate flame retardants and related mechanisms in H295R and MVLN cell lines and in zebrafish. Aquat. Toxicol. 114-115, 173-181 (2012).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for 11-keto Testosterone ELISA Kit

View all analysis workbooks

View the pre-configured analysis tool for 11-keto Testosterone ELISA Kit on MyAssays.com

Contact Us
  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of 11-keto Testosterone ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640