Pricing updated 2018-02-19. Prices are subject to change without notice.
Angiotensin II is a primary reactive vasoconstrictor, the main stimulus for aldosterone release, and one of the causative factors of chronic hypertension. The active angiotensin II octapeptide is released via a tightly controlled series of prohormones and proteases as diagrammed below. Normal human plasma angiotensin II levels are from 10-
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Carroll, W.X.,Kalupahana, N.S.,Booker, S.L., et al. Angiotensinogen gene silencing reduces markers of lipid accumulation and inflammation in cultured adipocytes. Front. Endocrinol. (Lausanne) 4, 10 (2013).
Marampon, F.,Gravina, G.L.,Scarsella, L., et al. Angiotensin-
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Is the Angiotensin II EIA Kit suitable for use with rat plasma and supernatant of cell culture?
The sequence for angiotensin II is conserved across mammalian species. Therefore, this kit will work with rat samples, however purification is required.
Is purification needed for plasma samples prior to use in the Angiotensin II EIA Kit?
Yes, plasma samples need purification prior to use in the Angiotensin II EIA Kit. The chemicals are available from Sigma: o-Phenanthroline Catalog number 33510, p-hydroxymercuribenzoic acid Catalog number 55540 Pepstatin A Catalog number 77170. The phenyl cartridge is from Phenomenex/Strata. The catalog numbers are: 1 ml Cat 8B S006 EAK , 3 ml Cat 8B S006 HBJ.