7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit | Cayman Chemical
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7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

Item № 600120
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     1 ea $178.00 0.00

Pricing updated 2018-09-19. Prices are subject to change without notice.

Description
Features
  • Label target cells with highly fluorescent CFSE for easy identification
  • Dead cells stain with 7-AAD
  • Analyze by flow cytometry to assess target cell killing
Synonyms
  • ​7-Aminoactinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester​
  • 7-Amino Actinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester

Cayman’s 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit employs CFSE to label target cells within the mixed cell population and 7-AAD to label dead cells. This kit provides an improvement over the traditional 51chromium (51Cr) release assay to assess cell-mediated cytotoxicity. CFSE labeling is more sensitive, does not employ radioisotopes, and cytolysis can be assessed at the single-cell level. The mixed lymphocyte reaction is a cornerstone method of immunology1, and this kit can help immunologists to detect subtle changes in cytotoxic lymphocyte function. The kit provides sufficient reagents to effectively stain approximately 109 cells.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • ​7-Aminoactinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester​
  • 7-Amino Actinomycin D/5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Benczur, M., Petrányi, G.G., Pálffy, G., et al. Dysfunction of natural killer cells in multiple sclerosis: A possible pathogenetic factor Clinical and Experimental Immunology 39, 657-662 (1980).

Technical Support
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You may be eligible to receive a free sample of 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640