Methionine Sulfoxide Immunoblotting Kit | Cayman Chemical
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Methionine Sulfoxide Immunoblotting Kit

Item № 600160
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     10 blots $356.00 0.00

Pricing updated 2018-12-14. Prices are subject to change without notice.

  • Detect proteins containing MetO residues by Western blot
  • Positive control included
  • MetO

Protein methionine sulfoxide (MetO) is a reversible oxidative modification that occurs by exposure of protein(s) methionine residues to reactive oxygen species (ROS).1,2 Methionine oxidation can alter the function(s) of the modified proteins and if not reversed by MetO reductases can be further oxidized to methionine sulfone, an irreversible modification.3 Methionine oxidation may occur as a by-product of phagocytotic oxidative bursts, normal mitochondrial respiration and may also occur from environmental chemical exposures.1,4,5 The overabundance of methionine sulfoxidation is implicated in age-related diseases.4 Cellular protein MetO levels may be influenced by the decrease or loss of methionine sulfoxide reductase (MSR) activity or by an overabundance of ROS leading to increased levels of dysfunctional proteins.5,6 The Methionine Sulfoxide Polyclonal Antibody provided in this assay kit detected IgG-containing MetO from sera of Alzheimer’s patients but not from normal control sera.2 Cayman’s MetO Immunoblotting Kit contains reagents needed for the immunochemical detection of proteins containing MetO residues by western blotting. MetO-containing samples of interest include those from cell or tissue lysates as well as semi-pure or purified proteins. Samples may be prepared with reducing or non-reducing sample buffer prior to SDS-PAGE and tested along side one SDS-PAGE well designated for the provided positive control. The MetO Polyclonal Antibody was isolated from rabbit serum generated after immunization with a oxidized corn protein (MetO-DZS18) rich in methionine.2 This polyclonal antibody is specific for protein methionine sulfoxide.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • MetO
  • Animal/Bovine
  • Animal/Rabbit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Stadtman, E.R., Van Remmen, H., Richardson, A., et al. Methionine oxidation and aging Biochimica et Biophysica Acta 1703, 135-140 (2005).

2. Oien, D.B., Canello, T., Gabizon, R., et al. Detection of oxidized methionine in selected proteins, cellular extracts and blood serums by novel anti-methionine sulfoxide antibodies Archives of Biochemistry and Biophysics 485, 35-40 (2009).

3. Moskovitz, J., Jenkins, N.A., Gilbert, D.J., et al. Chromosomal localization of the mammalian peptide-methionine sulfoxide reductase gene and its differential expression in various tissues Proceedings of the National Academy of Sciences of the United States of America 93, 3205-3208 (1996).

4. Berlett, B.S., and Stadtman, E.R. Protein oxidation in aging, disease, and oxidative stress The Journal of Biological Chemisty 272(33), 20313-20316 (1997).

5. Cabreiro, F., Picot, C.R., Perichon, M., et al. Overexpression of mitochondrial methionine sulfoxide reductase B2 protects leukemia cells from oxidative stress-induced cell death and protein damage Journal of Biochemistry 283(24), 16673-16681 (2008).

6. Levine, R.L., Mosoni, L., Berlett, B.S., et al. Methionine residues as endogenous antioxidants in proteins Proceedings of the National Academy of Sciences of the United States of America 93, 15036-15040 (1996).

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