NAD/NADH Cell-Based Assay Kit | Cayman Chemical
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NAD/NADH Cell-Based Assay Kit

Item № 600480
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CART TOTAL
     1 ea $295.00 0.00

Pricing updated 2018-08-14. Prices are subject to change without notice.

Description
Features
  • Measure total cellular NAD
  • Assay 38 samples in duplicate
  • Assay Range: 15.6 nM to 1 µM
  • Plate-based colorimetric measurement (450 nm)
Synonyms
  • Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide, reduced

Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman’s NAD/NADH Cell-Based Assay Kit provides a colorimetric method for measuring intracellular NAD+ and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
Synonyms
  • Nicotinamide adenine dinucleotide/Nicotinamide adenine dinucleotide, reduced

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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  • Direct Phone: (734) 975-3888
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You may be eligible to receive a free sample of NAD/NADH Cell-Based Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640