GLP SAM-Screener™ Assay Kit

Item № 600570
384 wells $375.00 0.00
1920 wells $1,400.00 0.00

Pricing updated 2018-02-22. Prices are subject to change without notice.

  • Homogeneous fluorescence polarization assay to screen for SAM-binding site inhibitors
  • Designed for high-throughput screening
  • Plate-based fluorescence polarization measurement (ex 575 nm, em 620 nm)
  • G9a-like Protein

Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of the epsilon amino group of lysine. G9a-like protein (GLP) is a SET domain-containing methyltransferase that specifically mono- and di-methylates histone H3 at lysine 9 (H3K9). GLP and G9a function as major euchromatic H3K9me1 and H3K9me2 histone methyltransferases and also have been found to methylate several nonhistone substrates, including p53(K372). This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in GLP. Binding of the small molecule probe to GLP induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The GLP SAM-Screener™ Assay is robust (Z' >0.5) and exhibits a greater than 100 mP shift over a range of 0-250 nM GLP. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1,536-well) if desired. *United States Patent 9,120,820

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • G9a-like Protein

Warning - this product is not for human or veterinary use.

Shipping & Storage
Dry ice in continental US; may vary elsewhere
≥ 1 year
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Cayman Chemical

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Ann Arbor, Michigan 48108 USA

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