Neutrophil (mouse) Isolation Kit | Cayman Chemical
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Neutrophil (mouse) Isolation Kit

Item № 601070
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL
     1 ea $195.00 0.00

Pricing updated 2018-11-19. Prices are subject to change without notice.

Description
Features
  • Isolate mouse neutrophils from peritoneal lavage or bone marrow
  • Circumvents issues common to isolating neutrophils from mouse blood
  • Includes a Percoll® density separation gradient
  • Analyze purified neutrophils by flow cytometry

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and beta-defensins. Neutrophils also form extracellular traps, web-like structures composed of DNA, histones, and various antimicrobial proteins that are extruded from the neutrophil in order to ensnare and kill extracellular pathogens. In mice, neutrophils comprise ~10% of circulating white blood cells, so unlike humans, it is usually not possible to isolate even one million neutrophils from a single mouse from peripheral blood. Cayman’s Neutrophil (mouse) Isolation Kit provides a streamlined method for isolating mouse neutrophils by peritoneal lavage. Included in this kit are all the reagents necessary for inducing a sterile inflammatory response in the peritoneal cavities of up to five mice, the density separation medium required to separate the neutrophils from other peritoneal leukocytes, and the fluorochrome-labeled antibodies required to assess the success of the isolation procedure by flow cytometry.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information

Warning - this product is not for human or veterinary use.

Shipping & Storage
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Stability
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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You may be eligible to receive a free sample of Neutrophil (mouse) Isolation Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640