pH-XtraTM Glycolysis Cell-Based Assay Kit

Item № 601230
96 wells $350.00 0.00

Pricing updated 2018-02-25. Prices are subject to change without notice.

  • Real-time fluorometric measurement of extracellular acidification associated with glycolysis
  • Includes control compounds to increase or decrease glycolytic rate
  • Adaptable to high-throughput screening
  • ECAR Assay Kit
  • Extracellular Acidification Rate Assay Kit

Cayman’s pH-Xtra™ Glycolysis Cell-Based Assay Kit is designed to measure the extracellular acidification rate (ECAR), which is proportional to the rate of lactate production.1 This kit combines a glucose-based respiration buffer and inhibitors along with the fluorescent pH probe pH-Xtra™ allowing for the accurate determination of glycolytic rate in a high-throughput manner.2

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • ECAR Assay Kit
  • Extracellular Acidification Rate Assay Kit

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 1 year
Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

References & Product Citations
Product Description References

1. Hynes, J.,O'Riordan, T.C.,Zhdanov, A.V., et al. In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assay. Analytical Biochemistry 390(1), 21-28 (2009).

2. Hynes, J.,Natoli, E., Jr. and Will, Y. Fluorescent pH and oxygen probes of the assessment of mitochondrial toxicity in isolated mitochondria and whole cells. Curr.Protoc.Toxicol. 2.16.11-12.16.22 (2009).

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You may be eligible to receive a free sample of pH-XtraTM Glycolysis Cell-Based Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

Toll Free: (800) 364-9897

(USA and Canada Only)

Fax: (734) 971-3640